**2. Materials and methods**

### **2.1 Cell line and culture conditions**

Human cervical cancer cell line (HeLa) cells were obtained from American Type Culture Collection, HeLa (CCL-2). The cells were cultured in a 25-cm<sup>2</sup> flask following Minimum Essential Medium (Sigma, MEM) containing 10% FBS (Gibco Lab.), penicillin (100 unit/ml), and streptomycin (50 mg/ml). The cells were incubated at 37°C in a humidified %5 CO2 incubator [29].

### **2.2 Plant material and extraction**

Corms of *Colchicum baytopiorum* (ISTE: 81438, Antalya-Termessos, July 5, 2005) and *Colchicum umbrosum* (ISTE: 85333, Bolu-Abant, June 25, 2008) were dried. All dried plant materials were extracted with methanol. Methanol extracts were evaporated in a rotavapor. Plant extracts were prepared in five different concentrations (0.01 mg/ml, 0.05 mg/ml, 0.1 mg/ml, 0.5 mg/ml, 1 mg/ml) in MEM, supplemented with %10 FBS. Prepared concentrations were treated to HeLa cells in time period of 24, 48, and 72 h [30, 31].

*In Vitro Cytotoxic and Apoptosis Induction Potential of Two Plant Extracts on HeLa Cells DOI: http://dx.doi.org/10.5772/intechopen.105696*
