**9. Conclusion**

Techniques for evaluating the biocompatibility of nanostructured is not fully comprehensive since it is a continuously developing field where biomaterials are

*Toxicity Evaluation and Biocompatibility of Nanostructured Biomaterials DOI: http://dx.doi.org/10.5772/intechopen.109078*

being constantly developed for tissue engineering applications in regenerative medicine. Extensive use of nano biomaterials in regenerative medicine necessitates efficient techniques for evaluating toxicity at an affordable cost. Literature analysis clearly states that the cytocompatibility of biomaterials influences the output of in vitro experiments. The determination of parameters, such as cell type and nanoparticle concentration, required a detailed understanding of anticipated nanoparticle exposure and its metabolic activity within the human body. Hence selecting suitable methods for the analysis of in vitro toxicity will provide a proper idea about nanomaterials toxicity mechanism and can be effectively used in regenerative medicine.
