**Abstract**

Biosynthesized silver (Ag) and gold nanoparticles (Au NPs) were synthesized by using *Punica granatum* and *Citrus reticulata* extracts and these extracts act as stabilizer. The average sizes of the Silver and Gold NPs were in the range of 8–10 nm and 30–40 nm, respectively. The confirmation of NPs was done by UV-visible absorption spectra, X-ray diffraction (XRD), transmission electron microscope (TEM), and Fourier transform infrared spectra (FTIR) techniques. The luminescence studies of europium and samarium complexes with NPs were studied. Emission intensities of complexes with the presence of silver enhanced and quenched with gold due to the reabsorption. The toxicity and antimicrobial studies of nanostructures were studied.

**Keywords:** eco-friendly green synthesis, metal nanostructures, luminescence, MTT assay, antimicrobial activity
