**3. Conclusion**

Cytotoxicity assessment is an important assessment in pharmaceutical and environmental industry to produce novel drugs and identify potentially harmful substances. In recent years, experimental methods to evaluate cytotoxicity have improved due to the progress of modern biology. However, there is no uniform cytotoxicity assay and the most popular methods currently used still entail tracking changes in cell morphology, cell proliferation and differentiation by labelling cells with colorimetric or fluorescent dyes that target compromised membranes of dead cells or specific organelles in live cells. Trypan blue dye is the most common dye used in cytotoxicity assays however due to its lack of specificity and sensitivity, it should be made mandatory to counterstain cells with dyes like neutral red, acridine orange and propidium iodide to provide a robust result. This would help eliminate potential drastic patient outcomes that may result from pushing a toxic compound into the market.
