**2.5 Statistical analyses**

Firstly, all data were checked with a normality test and then analyzed under a general linear model, using the GLM procedure of SAS v. 9.4 (SAS Institute Inc., Cary, NC). The means were determined using the PDIFF option of SAS, and Tukey's multiple range tests were used to compare means between SV and SF. Statistical differences were declared at *p* < 0.05, and tendencies at *p* < 0.10.
