**13.3 Polymerase chain reactions (PCR)**

Polymerase chain reaction is a laboratory technique for amplifying millions to billions of copies of a fragment of DNA. PCR involves using short synthetic DNA fragments called primers to select a segment of the genome to be amplified. To identify the species from the DNA samples, the targeted sequences can be amplified using universal or species-specific primers following the forward and reverse primer; 1x Buffer; 25 mM MgCl2; 100 mM dNTP; 25x BSA; 1 U/ul taq; template DNA. The PCR program is to be set in the thermocycler.
