**12. Wildlife sample collection and documentation**

#### **12.1 Wildlife sample collection kit**

The investigator must have a forensic sample collection kit to collect and store the biological materials. While in the field, the items required for sample collection are gloves, mask, datasheet, sterile vials, Ethanol or spirit, forceps, scissor, measuring tape, silica, envelopes, ziplock, cello tape, permanent marker, surgical blade, collection card (**Figure 1**).

#### **12.2 Collection and storage of biological samples**

Proper collection and storage of biological samples for the molecular analysis is very important known as biological resources. So, the proper preservation of biological sample is crucial because the preservation and laboratory analyzing at a future date. The processing protocol that will give quality result for the intended laboratory analyses must be selected from among various possible protocols. To collect blood samples, blood can be stored in vacutainers using sterile syringe and stored at 4°C for examination. Sometimes, blood spot or stains might be present during field investigation. The FTA classic card a commercially available product can be suitable for isolation, purification and storage of DNA samples. A drop of blood can be loaded on the specific FTA card. The fresh tissue samples can be stored in vials using molecular grade ethanol (100%). It can be labeled using marker and stored at −20°C. DNA in dried specimens ordinarily remains in good condition for at least a year. Frozen specimens AT -80°C will be remains stable indefinitely (**Figure 2**).

**Figure 1.** *Wildlife sample collection kit.*

**Figure 2.** *Collection and storage of biological samples.*
