**3. Diagnostic available tests**

There are direct and indirect methods to detect a Toxoplasmosis infection:

## **3.1 Direct methods**

They are based on identifying the presence of *Toxoplasma gondii* parasite or their nucleic acid. The techniques within these methods are fresh observation, histological stains, histopathological analysis, PCR, PCR real time, parasite isolation.

The materials available to use are newborn blood, umbilical blood cord, placenta, amniotic fluid, LCR, vitreous humor, biopsies, and other fluids [1, 8].

*3.1.1 PCR*

It is based on detecting the parasite nucleic acid using the polymerase chain reaction.
