**5. Conclusion**

The experimental strategies implemented in this work reproduced *in vitro* the natural cellular microenvironment necessary to establish the enteric development of *T. gondii* in the definitive host, the domestic cat. The primary feline intestinal epithelial cell culture indicated the potential contribution to new approaches for the investigation of parasite cell biology. We demonstrated that analysis of FIECs is an alternative method that could be used to understand the enteric cycle of *T. gondii* under controlled conditions, thereby opening the field for an investigation into the molecular aspects of this interaction. This approach could contribute to the development of new strategies aimed at intervention targeting one of the main routes by which toxoplasmosis spreads.
