**13. Conclusion**

Briefly, this chapter describes the involvement of DsbA in the pathogenesis of *P. aeruginosa*. We describe *dsbA* gene characterization with gentamycin cassette mutagenesis. The effect of disruption of the *dsbA* gene on virulence factor production is provided. More recently on biofilm formation as well. Since *P. aeruginosa* is an important pathogen we are constantly developing strategies to identify ways to eliminate it. A series of novel spirothiazolopyridine derivatives were designed and prepared with strong antimicrobial and antifungal properties [53]. Such compounds are likely to enhance our ability to stay abreast of AMR and novel pathogens. In addition, novel diagnosis methods being developed should be used to assess microbial contamination through innovative methods of diagnoses [54]. This collection of studies of Dsb proteins from different bacteria clearly shows their strong involvement in bacterial pathogenesis. We propose a more defined focus on the studies of these proteins and the development of methods to suppress their function. We also show a direct interaction of AlgT with the *dsbA* promoter a so far unique regulation not seen in gram-negative bacteria. Since Dsb proteins might be involved in biofilm we suggest a focus on their role in biofilm. We also suggest study of phenazine compounds like pyocyanin due to their role in bacterial pathogenesis [55]. It would be fascinating to see the involvement of DsbA in phenazine compound secretion and their redox status.

### **Acknowledgements**

I would like to thank my Ph.D. Mentor Dr. Dennis E. Ohman, Chair Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA, USA. He gave all the care and encouragement for the development of this stress response regulon hypothesis and the use of proteomics to identify components of this pathway. A similar proteomic strategy was used by other members of the Dennis Ohman Laboratory who contributed to my work through helpful discussions. I am thankful to Dr.(s) Sang Jin Suh, Laura Suh, Lynn Wood, Sumita Jain, Kerain Grande and Joanne Lee Johnston. Further thanks to members of my family, parents and brother who supported me through tough times of my Ph.D. research work. A special thanks to close friends like Dr. Gobardhan Das and Dr. Arshad Jilani. A special thanks to join me on this book chapter n to Dr. Sunil Kumar contributed to the section on Eukaryotic PDIs. His insight into their biology is appreciated.

*The Role of* Pseudomonas aeruginosa *DsbA-1 in Bacterial Pathogenesis: Current Research… DOI: http://dx.doi.org/10.5772/intechopen.108072*
