**3.4 Centrifugation**

After centrifugation, the yield of the cells was 0.7–0.8% pellet from 5 L LB broth. Pellets were pinkish in color with smooth cream (**Figure 4**). The pellets CFUs showed 1.0×1010/ml.

#### **Figure 1.**

*a)* Pseudomonas fluorescens *colonies in LB plate; b)* Pseudomonas fluorescens *showed gram-negative rod shape bacilli in gram staining.*

**Figure 2.** *CFU of mother inoculum.*

### **3.5 Lyophilization**

The lyophilized microbial powder (LP) was light brownish color and it showed 6% moisture during harvesting. The CFU of LP was recorded as 1.0×109 (**Figure 5**).

### **3.6 Formulation**

We analyzed dextrose formulations in two configurations, 5% and 10%. Both compositions showed 4.0×107 /gm (**Figure 6**) and 5.0×107 /gm (**Figure 7**), respectively, on the formulation day (**Tables 3** and **4**).
