**12. Future prospectus of microbiome**

Microbiome research generates a large quantity of data, which necessitates the use of advanced computational techniques, which are rapidly evolving. Furthermore, many of the existing mathematical tools analyze connection rather than causation. As a result, researchers should remember that microbiome characterization, data analysis, and modelling are only a small part of the discovery process, and that they should be used in conjunction with traditional in vitro and in vivo model studies to prove cause and effect. To advance microbiome research into the therapeutic domain, researchers must go beyond clinical association studies to validate their models in other clinical cohorts and understand the mechanisms of causation in vitro, ex vivo, and animal model systems. While metagenomic studies have revealed immense diversity, additional tools are required to understand the community structure, function, and their interaction with host environments. Using microbiome analysis with Next generation sequencing to help define biomarkers and stratify patient populations, which may help improve therapeutic outcomes in the future. In addition, gaining deeper understanding of the microbiome through improved tools and methods will enable engineers and innovators to develop better applications and unlock the potential of the microbiome. As a result, pre/probiotics are likely to be coupled with other dietary substances to generate a more powerful health benefit. Furthermore, merging multiple study disciplines and employing new technological approaches in microbiome research is predicted to open the way for the development of evidence-based clinical therapies for modern-day health challenges. Previous research has found that bacteria have 35.5 million functions, of which just 0.02% are known, according to computational predictions. Despite the growing body of research on the microbiome, our understanding of its function, particularly how it influences health and disease, is limited because to the lack of a "universal" standard for study comparability.

The microbiome has been the subject of significant attempts to unravel all of its genetic information for the benefit of humankind, which requires worldwide coordination. Ultimately, new clinical tools and applications should be developed, as each individual and every population requires more individualized and effective care.
