*3.3.11 Sucrose fermentation*

The sucrose fermentation test was performed to determine whether or not a microorganism had the capacity to ferment sugar. To conduct this test, the following substances were dissolved in 1000 ml of distilled water: 10 g peptone, 0.018 g phenol red, 5 g sodium chloride, and 5 g sucrose. The medium was then sterilized in an autoclave at 121°C for 15 min. After that, the medium was injected with bacterial culture. For 24 h, the test tubes were put in an incubator set to 37°C. If a yellow tint develops, it indicates that fermentation is taking place. Durham tubes were inserted in each test tube to observe gas generation [105].
