**3.8 Isolation of bacterial strains done by marketed foods and drugs**

Liu et al. [114] assesses 41 lactic acid strains, 36 of which have been identified and obtained from the commercial produced milk and pharmaceutical products, and 5 forms of probiotics were assesses that were obtained from China. These samples then incubate in the incubator for the period of 2 days in the conditions of anaerobically provided medium at the temperature of 37°, so different colonies have been morphologically chosen and that were classified as rods or cocci under a light microscope. After being plated on suitable agar plates, pure colonies have been isolated. The conventional microbiological techniques of colony appearance, gram staining, oxidase, and catalase reactions were initially used for all isolated isolates. Amplification of the PCR and further sequencing of 16S rDNA at genus level have been carried out. For identification purposes, universal primers 27F and 1492RP were employed. AGAROSE Gel was then separated and purified by electrophoresis by 1.5% (w/v). ABI DNA Sequencer 3730 was used to the purified products. The alignment of 16S rDNA was done by BLAST.
