*3.6.2 Antimicrobial production*

Bilkova et al. have evaluated the antimicrobial production of the chosen isolate. As an indicator strain E. coli was utilized. The supernatant culture was caught at the time period of different intervals consist of 21, 24, 27, 30 h of inoculation following intervals. Above supernatant the proteinase, followed by heat treatment, was centrifuged, and digested. It was put into 200 μl wells in E. coli lawn containing plates and incubated with control (sterilized water) for 37°C for 48 h to check for the presence of the indicator strain growth inhibition zone.
