**Abstract**

To increase the performance of diesel engine and environment, the utilization of biofuel as a major source of renewable energy is justified. It is well understood that agri-based biofuel is always also the choice in case of utilization as biofuel because of requirement of processing and threat to food security. Therefore, scope of improvement increases as it is yet to efficiently exploit as major full in the world. There are numerous factors that influence the efficiency of a fuel and its combustion. The physiochemical properties, namely viscosity, surface tension, flash point, latent heat of vaporization, oxidation, etc., allow the fuel to work efficiently during combustion. Thus, interests in biofuels have been increased, and various experimental studies have been developed for diesel engines consisting of methanol and methyl ester of rapeseed oil. In a relevant study, to achieve ideal biofuel, various biotechnological advances at the frontiers of plant science to dissect the underlying traits for identification of fatty acid profile useful for oil production and quality are essential, thereby ensuring food security. The plantbased fuel and its efficient utilization depend upon its oil quality and quantity, which thereby can be evaluated and enhanced by various conventional and nonconventional approaches of engineering and plant sciences.

**Keywords:** biofuel, combustion, fatty acid profile, physiochemical, biotechnological
