*2.1.1.2 Ipt gene from Agrobacterium tumefaciens*

The introduction of *Isopentenyl transferase gene* (*ipt*) isolated from *Agrobacterium tumefacien* encoding a key rate limiting enzyme in the cytokinin biosynthetic pathway into the tobacco with wound inducible promoter recorded reduced consumption of leaves by the tobacco hornworm (*Manduca sexta*) and reduced survival of the peach potato aphid (*Myzus persicae*) leaving negative effects on plant development, such as an underdeveloped root system and a reduced total chlorophyll content [21].
