**2.9 Mutation breeding**

Mutation breeding refers to the technique of developing and using genetic variation *via* induced mutagenesis. It is an effective method for improving complex traits, especially in crops with limited genetic bases, vegetative reproduction, and selfpollination. More than 3000 mutant cultivars of various crops have been published in more than 60 countries; of these, 776 mutants have been generated for various nutritional quality traits, including minerals [110]. When compared with wild-type radish, mutants displayed a 30% higher net photosynthetic rate and a 36% higher total chlorophyll content.
