**6. Conclusion**

The imperativeness of characterizing bioactive protein from root vegetables has been scientifically established beyond the acknowledgement of their therapeutic benefit. With the continued advancement in the available biophysical and biochemical techniques, more elucidation and insight into the factors that contribute to or affect their functionalities, structural orientation, and stability will continue to be available for researchers, with the intent they can be further improved on and developed as biopharmaceuticals. The various characterization principles and techniques that researchers have employed so far are with the view of ascertaining the authenticity of the nutritional and physiological content cum characteristics of root vegetables' bioactive protein, and this cannot be complete without biophysical and biochemical characterization. This is so as protein's higher order structure which can either be affected by environmental changes in a particular way, or when it interacts with other molecules such as ligands, denaturant, glycosylation, and oxidation states, remains a critical parameter in the development of bioactive protein into therapeutics.
