**6.4 Regulation of specific metabolism and transcription factor modulation**

The functional characterization of TFs involved in the control of anthocyanin metabolism is an outstanding example of how transcriptional regulatory research can be carried out to fine-tune specialized metabolic pathways. The WD-repeat/bHLH/ MYB complex regulates anthocyanin accumulation in plants by positively regulating the gene expression of DFR, anthocyanin synthase, and glucosyltransferase. This molecular pathway, conserved across many species via orthologous TFs, which is essential in the color determination of flowers and fruits such as those of apples, grapes, and oranges [141].
