**5.4** *B. napus L.'s* **efficient oil production is controlled by targeted mutation of**  *BnTT8* **homologs**

*B. napus* is an important oil crop, but despite its importance, no naturally occurring or artificially produced yellow seed germplasms have been discovered yet. Recently, in one of the studies, CRISPR/Cas9 system has been used to produce yellow mutant rapeseeds (*Brassica napus*). The targeted alterations of the *BnTT8* gene were persistently passed down through generations, and a variety of homozygous mutants with damaged alleles of the target genes were acquired for phenotyping. *BnA09*.*TT8* and *BnC09*.*TT8b* are the two targeted mutants of *BnTT8* which were able to restore the yellow-seeded phenotype. These mutants generated seeds with increased protein and oil content, improved fatty acid (FA) composition, and no significant abnormalities in yield-related parameters [133].
