**6. Conclusions and future prospect**

Since its emergence, RT-qPCR was the only approved gold standard molecular diagnostic technique that detects SARS-CoV-2. However, once studies started to utilize the colorimetric RT-LAMP for a faster and cheaper alternative, they proved the success of this technology in diagnosing COVID-19. Hence, some developed RT-LAMP SARS-CoV-2 detection kits received the approval by the FDA like the AQ-TOP™ COVID-19 Rapid Detection Kit, the Color SARS-CoV-2 RT-LAMP Diagnostic Assay, FastProof™ 30 min-TTR SARS-CoV-2 RT-LAMP, or the Lucira COVID-19 All-In-One Test Kit Labeling for emergency use authorization (EUA). Thanks to its simplicity and accessibility for end-users, this technology can be easily adapted for custom-use. For instance, Davidson and colleagues developed a paper-based colorimetric assay that contains lyophilized reagents for room temperature storage and distribution [18]. Also, some colorimetric RT-LAMP reagents, especially enzymes, can be made in-house to reduce the cost of the assay. To improve the efficiency of the detection, RT-LAMP can be combined with other technologies like CRISPR [35], nanotechnology [36], and many more. Therefore, the colorimetric RT-LAMP method must be further investigated not only for the detection of SARS-CoV-2, but also for broader applications such as human, plant, or animal-hosted viral pathogens.
