**2.1 Phylogenetic analysis**

BLAST searches were carried out to identify different procaryotic ortholog of the *N. gonorrhoeae* (GenBank accession number EEZ44675.1) KARI protein

which is conserved in *Neisseria* (https://www.ncbi.nlm.nih.gov/ipg/EEZ44675.1). KARI sequences were aligned using ClustalW [21], and then a neighbor-joining tree was generated using MEGA software [22]. KARI sequences from *Escherichia coli* K12 (AKD89606.1) *Enterococcus faecium* (KXH23108), *Staphylococcus aureus* (MBU4945389.1), *Klebsiella pneumoniae* (MBC4258974.1), *Actinobacter baumanii* (EHU1490884.1), *Pseudomonas aeruginosa* (EJY59157.1), were used for phylogenetic analysis.
