**2. Materials and method**

### **2.1 Location/study area**

Consolidated breweries plc Makurdi, Benue state is located at kilometer 5 Gboko Road, pm 102,339 Makurdi, Benue State, Nigeria. The locality is predominantly an agrarian community where farming and animal husbandry takes place with a few industrials such as small and medium scale enterprise (mechanic workshop, local markets, construction, and mining) activities. **Figure 1** shows the map of the study location.

### **2.2 Sample collection and preparation**

Four samples of effluent were collected with a cleaned plastic container at a different location in the brewery. The plastic containers used were carefully washed with 1% HNO3 acid, rinsed with tap water, and then distilled water. The samples were labeled appropriately; Sample A-untreated effluent, B- treated effluent, C- contact point of the treated effluent with the river, and D- 10 kilometers away from the contact point. These samples were transported to the laboratory for analysis in an icepacks container and protected from direct sunlight. They were stored in the refrigerator at 20°C.

**Figure 1.** *Study map showing the study locations.*
