**8.1 qPCR technology**

Event-specific PCR detection technology is commonly employed for GMO testing due to its ability to specifically detect each transgenic event simply by targeting their unique junction between the host genome and the transgenic cassette [51]. Currently, different event-specific qPCR (quantitative) technology has been designed for transgene detection from GM Corn, Cotton, Canola, Rapeseed, and rest of the crops [52]. The qPCR system, which is the most common strategy, allows detecting, identifying, and quantifying GMO via the SYBR Green or TaqMan chemistries in agricultural and food products [53]. Though, the (qPCR) methods have more reliable, accuracy and greater sensitivity but its success largely relies on various factors, e.g., its throughput strategy is often restricted to one marker per reaction. Due to continuous growth in GMO production, new/additional detection markers (for specific detection of new transgene) are required to be designed continuously and used to completely cover their identification. In case of multiplex PCRbased methods, several DNA targets can be detected in a single reaction.
