*Genetically Modified Food for Ensuring Food Security Issues DOI: http://dx.doi.org/10.5772/intechopen.106810*

in millions of sequencing reads [62]. NGS is an efficient tool for transgenic events detection even in the absence of sequence information of such events. NGS is being efficiently employed for characterization of site addition, flanking regions, accidental addition as well as the determination of transgene copy number [63]. Two main approaches are (targeted sequencing strategy) or (whole genome sequencing (WGS) strategy), for samples sequencing which has been enriched previously with desire sequence regions have been identified [64]. Molecular analyses of transgenic varieties from GM soy and GM rice have successfully been achieved using this strategy [65]. An approach based on initial enrichment of the targets using DNA walking starting from transgenic elements covering a large spectrum of GMO has been proposed to characterize unknown sequences including the transgenic inserts and junctions [66]. DNA walking technology will become more sensitive and more suitable and that could provide a more promising solution for the recent challenges of GMO analysis in the near future.
