**8.3 Loop mediated isothermal amplification (LAMP)**

Loop mediated isothermal amplification (LAMP) is an emerging technology which was developed an easy detection of the transgenic event in a given sample [57]. The main idea is the amplification and identification of the desired nucleic acid sequences at a steady temperature and at some specific stage of the experiment. This novel approach of GMO detection involves the utilization of four distinct primers which identify at least six different segments of the desired DNA. Varieties of LAMP markers were designed for quantitative detection of transgenic GM events [58]. The advantage of this study is simplicity, time-efficiency, and ability to withstand different PCR inhibitors, for instance, acidic polysaccharides. However, this technique has some limitations, e.g., designing four primers per sequence and detection of different GM events employing multiplex approach is also a problem.
