**5.4 Purification of protein**

Purifying specific proteins from an isolate or concentrate using chromatographic techniques is a helpful technique. Ion exchange, reversed-phase, and affinity chromatography rely on the variable affinity of proteins for the immobile stage [62, 67], and gel filtration chromatography isolates proteins by molecular weight [63]. Although chromatographic purification technologies have yet to be utilized on insects for food. For example, ion exchange chromatography was used to partially purify arginine kinase from yellow mealworms [68].
