**3.10 Accessing total genome of microbiota**

It is not yet universally accepted literature published for the extraction of metagenomics DNA from insects. The major goal is to access unbiased microbial genome of whole communities along with the contamination and degradation of the genome should be taken under consideration. In the DNA isolation the sheering or DNA damage should be taken with care so that the DNA with high molecular weight can be obtained, which can then be used to create DNA libraries through BAC vectors. The DNA should be free from downstream of the applications such as cloning and PCR so, for this purpose no macromolecules should be attached to DNA [95].
