**3.9 Cultivation of obtained microbiome on the culture**

The obtained gut sample is then suspended in saline, phosphate buffers, and then serially diluted to get cultured on the suitable growth medium. The culturing plates are then kept in incubator for 48 h. After this the morphological characteristics are carried for the characterization of bacterial colonies with at least three dilutions. Subsequently, enzyme activities are studied by gene coding for enzymes are cloned and DNA is sequenced for genomic libraries. The cultivated bacteria are then obtained and then used for the DNA extraction. Subsequently, enzyme activities are studied by gene coding for enzymes are cloned and DNA is sequenced for genomic libraries [94].
