**2.10 Cytokine analysis**

Detection of TNF-, IFN-, IL-10, IL-6, IL-17A, IL-4, and IL-2 was performed through BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine Kit (cat. 560,485; BD Biosciences, San Jose, CA) following manufacturer's instructions. Samples were acquired in the BD Q13 FACSCallibur flow cytometer (BD Biosciences) and data were analyzed with FCAP Array software (BD Biosciences).
