*4.2.1 Loop-mediated isothermal amplification (LAMP)*

Now, molecular diagnostic technologies based on nucleic acid amplification have been applied extensively in the detection regions, such as Loop-mediated isothermal amplification (LAMP) developed by Notomi [41–45]. Various confirmatory studies have been used to evaluate the feasibility of LAMP technology for microbial identification and diagnosis [42]. LAMP kits for detecting *Salmonella*, *E. coli,* and *Listeria monocytogenes* have been commercialized in the initial phase of development.

The loop-mediated isothermal amplification method offers several advantages: high sensitivity (2–5 orders of magnitude higher than conventional PCR methods); short reaction time (30–60 min can complete the reaction); no special instrumentation is required for clinical use; the operation is simple (whether DNA or RNA, the detection step is to mix the reaction liquid, enzyme, and template in a reaction tube, place in a water bath pot or incubator at 63°C for about 30 to 60 minutes, observe the results by the naked eye) [42–44]. There are also some disadvantages of the loopmediated isothermal amplification method: high sensitivity, easy to form aerosol pollution once the lid is opened, combined with the current majority of domestic laboratories can not strictly partition, false-positive problems are relatively severe, so we strongly recommend using real-time turbidimeter during the development of the kit, do not open the reaction tube after the reaction. Primer design is more demanding, and some disease genes may not be amenable to the use of loop-mediated isothermal amplification methods [41–43].
