**2.2 Regeneration medium of** *L. plantarum*

The regeneration medium used was solid MRS with the composition: MRS 5.22% w/v and agar 2% w/v. The materials were dissolved with distilled water up to 100 ml. Each was distributed as much as 4 ml into a tube and then sterilized by autoclaving at 121°C for 15 minutes with a pressure of 1 atm. The sterile medium is cooled to solidify to form slant agar.
