**Table 1.**

*All test carry out.*

facilitated that it's received a mean value of 9. 85 Klux. The luminous intensity was measured with a battery lux meter with three work ranges.

All the experimental series to laboratory scale got ready with distilled water, as diluent of the reagents that conform the synthetic medium. According to the applied reaction, was used commercial sugar cane like source of organic carbon; also for a specific culture molasses was used and for another, sodium bicarbonate (NaHCO3) to carry out a comparison among the different experiments, diverse culture medium were used with the unlike reagents that compose the Johnson medium in an individual way.

Of the obtained results, with the purpose of checking to a bigger scale the best results, was carried out an experiment to pilot plant scale with a lagoon that contained *Mixotrophyc Culture of* Dunaliella salina *in Cuban Fishing Wastewaters DOI: http://dx.doi.org/10.5772/intechopen.104803*

1000 liters of culture, whose dilution water was fresh water with addition of commercial salt until reaching a concentration of 20% (Weight/Volume).

Cell counts were determined daily by a Neubauer cell hemocytometer with 1 mm<sup>2</sup> of useful area. The pigments ß-carotene and chlorophyll a and b, was extracted with acetone and were determined according to the standard method of the ABL [5], in a cellular button after centrifuging during 8 minutes to 4500 r.p.m. A volume of 5 mL of blended cultivation with 5 mL of distilled water (H2O), for this way to diminish the salinity of the sample and to avoid that the cells that lack rigid cellular membrane break, being ignored this way the spill of the liquid, plasmatic to the water of the means. The calculations were carried out according to:

```
Total carotene = ABS452 * 3.86 (Vol. extract/Vol. it shows)
Chlorophyll to = 11.93 * ABS664 - 1.93 * ABS647
Chlorophyll b = 20.36 * ABS647 - 5.50 * ABS664
```
The primary data of the different series of growth in number and the ß-carotene concentrations did not resist a test of normality, and when relating the logarithm of the variances of each data series against the logarithms of the stockings of the cel/ mL counts, a pending "b" of 2.47 was obtained since all the values were normalized according to expression log10 (x + 1). The slope for the regression of the ß-carotene concentrations was 2.33; because the same transformation was applied to normalize the data. Total proteins were determinate following the method of Lowry et al. [6]. The dry weight was analyzed by gravimeter. The validity of the results was analyzed applying an analysis of variance (ANOVA) of double classification with Duncan and Tuckey tests using a significance level of 95% and were solved by means of the packages of programs StatWin 8.0 and Excel 2016.
