**4.4 An experiment with** *Spirulina* **and cancer cell lines**

The simplest experiment was performed with the help of *Spirulina* [28]. It was the key ingredient in the protocol. The easy methodology adopted was devised after considering cancer patients. The patients taking four cycles of chemotherapy were randomized into groups and categorized as "control" and "patient consuming the medication" [35]. In the first two cycles, the *Spirulina* was made to be consumed in 3 capsules of 100 mg each, 3 times a day [28]. The patients were taking their medications as normal, as prescribed. It influenced many things like it inhibits tumor growth interferon and interleukins like the *TNF-alpha* [35]. It works against HIV and increases the growth of CD4 cells and works on liver inflammation. The reduction of breast malignant is 87 to 13 percent [28, 39]. When the blood test was taken, the account of WBC and NEUTR. It was better with patients treated with *spirulina* and low for control [39]. The hemoglobin levels were approximately the same, whereas *myelosuppression* was lower in the treated patients rather than in the control group. IgM and CD8 were higher in treated patients [28]. The fact is that *Spirulina* slows down the *MAPK pathway.* When CD8 needs to be more numbered, as when it is exhausted, it increases apoptosis [39]. This was effective against *HepG2, MCF-7, AG49, HT29* which are liver, breast, alveolar basal epithelial cell, and colon cancer cell lines respectively [28]. A few limitations were also reported like the population size was comparatively small, and the effects after treatment were not observed [28]. There was a lack of evidence for treatment applied to the *neoplasm*. Observation and research were not performed at the molecular level [35]. Vitamin extract from the algale variant is effective for metastasis treatment and its effectiveness is presented in **Table 3**. Coenzyme Q: Ubiquinone in combination with ɑ-lipoic acid and cisplatin ceasees colon cancer cell line HCT116 [29].


**Table 3.**

*Vitamin extract from algal variant effective for metastasis treatment and effectiveness.*
