**5.3 Cultivation issues with microalgae**

The cultivation of microalgae is required on a large scale considering its potential benefits for developing the target medicine and other applications. The need for arable land, light, oxygen, pH and other climatic conditions is required [12]. They can grow in various places, but they will possess various properties and potential [12]. If a specific industry requires a specific compound extract that is only found in a particular area, then these conditions are mandatory to be fulfilled [28]. Now, considering this, the laboratory where attempts are made to cultivate them, but they show setbacks. The setbacks include not being able to provide all conditions, chemicals to some extent, and obviously, the technology which makes it even harder to process [9]. The ability to protect it from contamination is a tough task as well [70]. Proper instruments are not there to aid in this. Nature is incredible as the laboratories are trying hard. To take a step further large bioreactors have opted, but they are not getting beneficial results [12].

### **5.4 Lack of technology and awareness**

Cancer treatment requires more sophisticated technology. The invention is the mother of all innovations, so the technology has upgraded itself a lot. The biotechnology industries have grown so much because of the tools and medication [47]. The technology is an expensive brand, which costs a lot. It takes a lot for a technology to be launched and reach out to the people [50]. The technicians working on the prevention and treatment of cancer sometimes become clueless because proper protocol and complete research on a disease like cancer are still incomplete. The disease is an elaborative and extensive field that requires many aspects of treatment but is still not researched [51]. Technology and awareness are missing; therefore, the requirement is for a higher level that could diagnose cancer at the earliest stage, and surgery and chemotherapy should have alternative ways as well [36].
