*Mixotrophyc Culture of* Dunaliella salina *in Cuban Fishing Wastewaters DOI: http://dx.doi.org/10.5772/intechopen.104803*

experimental design as it is shown in the **Table 1**. As control the modified Johnson medium (MJ), was used [4], that is it usually used for the commercial culture of this microalgae. The employee inoculates belongs to the strain HFI-1; obtained in the MIP by sexual crossing among a strain from the Algal Culture Collection (ACC) of the "Centro Nacional de Investigaciones Científicas" (CENIC) in Cuba, whose origin is Chilean and another (MUR-8) from ACC at Algal Biotechnology Laboratory (ABL) of Murdoch University, Western Australia. The seeding, was carried out adding a seed of 300,000 cel/mL in erlenmeyers flask of 200 mL with 100 mL of salininzated medium with addition of sodium chloride (NaCl), until obtaining a concentration of 20%. The culture mother's cells were in the phase of exponential growth and Betacarotene production.

The incident illumination received on all the experimental flasks, was continuous, during all day, with fluorescent light tubes of 40 Watts and the rotation of the flasks


