*2.4.1 Methods*

Folin & Ciocalteu method [24] with catechin as a standard was used for analysis. Ethanol extracts of mushroom mycelia (1 mL) were mixed with 0.5 mL of Folin & Ciocalteu solution and 5 mL of 0.4 M sodium carbonate solution. The absorbance of the reaction mixture was quantified at 660 nm after the reaction was set to complete for 30 minutes at 30°C. Methods are described in detail in Suruga et al. [9].
