*4.2.1 Methods*

Yeast alpha-glucosidase inhibitory activity was measured using methods reported before [52] with modifications as described in Suruga et al. [10]. Briefly, yeast alpha-glucosidase was incubated with fermented soybean extract solutions and then p-nitrophenyl α-D-glucopyranoside (pNP-glucoside) was added and absorbance was determined at 400 nm. For the mammalian reaction, alpha glucosidase from rat

*Medicinal Mushroom Mycelia: Characteristics, Benefits, and Utility in Soybean Fermentation DOI: http://dx.doi.org/10.5772/intechopen.102522*


**Table 1.**

*Total phenolic content and antioxidant activity of soybeans fermented by mushroom mycelia [10].*

intestinal acetone powder [53] was incubated with fermented soybean extract and the amount of glucose released was measured. We also used maltose as the substrate and calculated the % inhibition rate of alpha glucosidase [54].
