**9. Quinoa-based protein concentrate**

The process of obtaining protein concentrate based on quinoa will be shown below [50]:

### **9.1 Sample preparation**

The grain was cleaned and classified, subsequently a washing was carried out with distilled water and followed by drying at a temperature of 75°C for 8 hours. Furthermore, the saponin-free grains were ground, and a particle size of 25 μm was reached with a sieve.

#### **9.2 Alkaline solubilization**

A flour suspension was made, using distilled water with a 1:10 (w / v) ratio, and the pH is regulated with 1 N NaOH until reaching pH 9. Additionally, this suspension was moved for a time of 1 hour, the supernatant was taken (first extraction (and the precipitate was re-carried out in the solubilization process (second extraction).

*Simulation*

 *flowsheet designed with SuperPro Designer 9*

*® for the QPH production*

 *process using (a) SFE and (b) CSE.*

*Nutritional Value, Methods for Extraction and Bioactive Compounds of Quinoa DOI: http://dx.doi.org/10.5772/intechopen.101891*

#### **9.3 Isoelectric precipitation**

From the previous step, the collected supernatants, their pH were regulated with citric and hydrochloric acid until reaching a value of 4.5. The samples were centrifuged for a time of 15 minutes. Subsequently, the supernatant was discarded and with the precipitate, they were lyophilized at a temperature of 30°C. Finally, the samples obtained were placed in polyethylene material sleeves.
