*6.3.2 Harvesting of CE cells*

Prior to trypsinization using 250 μl of 0.1% trypsin 1:250 (Invitrogen, Canada, Cat # 27250-018) and 0.5 mM EDTA (Life-Technologies, USA, Cat # 15576-028) solution (made in sterile 1 PBS and sterilized by 0.22 m syringe filter) for 2–3 minutes, the cultures were immediately rinsed twice in pre-warmed PBS before being used to harvest the cells. To stop the trypsinization, 750 μl of growth media was added. Centrifugation at 500 g (2400 rpm) for 10 minutes at room temperature (25°C) was used to collect the cells. 750 μl of supernatant was discarded and remaining 250 μl along with cells pellet was preserved at 40°C for RNA isolation.
