**1. Introduction**

The strawberry (*Fragaria spp*.) is one of the most popular berry fruits in the world. Strawberries contain high levels of vitamins C and K, as well as fiber, folic acid, manganese, and potassium [1]. They are also rich in phytonutrients and flavonoids, which give them their bright red color. They have been used medicinally for centuries to help with digestive problems, tooth whitening, and skin irritations. Their high fiber and fructose content may help control blood sugar levels by slowing digestion, and the fiber is thought to have a satiating effect. Leaves can be eaten raw, cooked, or used to make tea [2–5].

In Thailand, strawberries have been grown for many years in the north of the country and have become economically important since the early 1980s. The Royal Project Foundation's research resulted in the creation of a popular cultivar known as "Royal 80". This cultivar has a pleasant flavor, is early to bear fruit, and is of good quality. The absence of good mother plants for runner plant production is an issue in strawberry farming in Thailand. There are very few virus-free plants available, and plant vigor is low [6, 7]. The propagation of strawberries using tissue culture technique is one way to solve this problem. However, production costs are high due to the expensive chemicals used for the propagation recipes [8]. To reduce the high expenses of the tissue culture technique, we are interested in using chemical fertilizers, which farmers used for strawberry production instead of MS medium. Farmers for strawberry production use the strawberry fertilizers such as 21-21-21 (N-P2O5-K2O), 15-30-15 (N-P2O5-K2O), 36-5-5 (N-P2O5-K2O), 15-30-15 (N-P2O5-K2O)**,** and 10-52-17 (N-P2O5-K2O). Therefore, developing recipes to reduce production costs is necessary in conducting this research. So far, there is no report on the use of fertilizers to substitute strawberry production medium. The aim of this study was to investigate the effect of types and concentration of chemical fertilizers by using five types of replacement mediums on *Fragaria* spp. to reduce production costs.
