**8. Conclusion**

In order to detect more stable QTL regions in plants, it is basically needed to have a high-quality genome assembly. By increasing the quality of the octoploid strawberry

genome, it can be ensured that the four sub-genomes are brought together or differentiated with a good assembly. In addition, better coverage of individuals' genomes will be ensured with next-generation SNP sequences. In this way, it will allow a more accurate description of the genome functions of the relevant QTL regions. It will also allow the discovery of additional chromosomal regions that control economically important fruit traits and the development of functional markers targeted by candidate genes. Their high-resolution mapping along with the reduction of unit cost in sequencing will allow the discovery of rare markers associated with economically important traits.

Recently, a quality genome assembly and the mapping of QTLs associated with economically important traits and their use in MAS have greatly facilitated breeding programs. To date, most of the QTLs detected are environment specific. The number of experiments, the year, and the application and cultivation conditions are very important for QTL studies. In particular, the selection of populations such as F2, F3, and BC to be used for mapping is very important, as are other conditions. The distance of associated QTLs, the impact of major QTLs on the population, environmental factors, population size used in mapping, and based on individual errors affect the accuracy of QTLs. However, increasing the number of individuals in the population allows the estimation and determination of the phenotypic variance value of minor QTLs. On the other hand, the techniques used for the validation of QTLs are very important for the use of linked markers in MAS. Some important loci may be eliminated due to the selection of inadequate verification methods of linked markers. Therefore, the discovery of QTLs associated with economically important traits in strawberries for selection at an early stage is very significant. In this process, this summary can be useful for QTLs linked to strawberry traits.
