**2.2 Statistical analysis**

The experiment carried out in 5 **×** 4 Factorial in CRD (Completely Randomized Design) consisted of two factors as factor A was 5 types of chemical fertilizers as 21-21-21 (N-P2O5-K2O: Procon), 36-5-5 (N-P2O5-K2O: Superspeed), 30-20-10 (N-P2O5- K2O: Plalungchang pink), 15-30-15 (N-P2O5-K2O: Megafor), 10-52-17 (N-P2O5-K2O:

**Figure 1.** *Stolon of strawberry cultivar royal 80 was used to be an explant.*

Plalungchang blue) and MS as a control, factor B was 4 levels of chemical fertilizer concentration as 0.5, 1.5, 2.5, and 3.5 g L−1. The data was analyzed statistically by Statistix 8 program and the significance of differences among means was conducted using LSD at p ≤ 0.05 and p ≤ 0.01. Data was recorded after 4 weeks of culture.
