**Abstract**

The cultivated strawberry (*Fragaria* x *ananassa*) is derived from *Fragaria chiloensis* and *Fragaria virginiana* species a few centuries ago, and it is one of the most preferred and consumed berries all over the world because of its a good source in terms of many nutritional elements. Strawberry has high genetic variability and adaptation to different environmental conditions due to its highly heterozygous nature. In the last decades, many farmers, breeders, researchers even consumers have started to focus on berry quality traits such as large fruit, uniform shape, high fruit firmness, high fruit sensorial quality (aroma contents), color, gloss, and resistance to pathogens. Thus, the development of novel strawberry cultivars or genotypes with high nutritionally quality traits has become one of the main aims in strawberry breeding programs. Biotechnological tools such as the identification of quantitative trait loci (QTL) and marker-assisted selection (MAS) are the most widely used technologies in fruit breeding programs for shortening the breeding period. Identification of QTLs in agnomical important traits are very valuable tools for early selection in strawberry breeding programs. This chapter is focused on QTL and marker assisted breeding studies in strawberry to date and provides new perspectives on molecular breeding in strawberry breeding.

**Keywords:** strawberry, QTL, NGS, SNP
