**6. Conclusions**

The development of new elite cultivars with high yield, quality, and biotic and abiotic resistance or tolerance, will improve the efficiency of *Fragaria* production. In addition, the use of virus-free plant propagules may also increase yields and limit the spread of viral diseases within and between countries. Therefore, *Fragaria* germplasm conservation and its back up is of utmost important.

For a cryopreservation protocol to be successful or to provide optimal results, experimental conditions for each of its successive steps must be optimized. The protocols stated in this study need to be optimized for better regrowth. There is scope for improvement at each step of the tested techniques, to obtain higher recovery of cryopreserved shoot tips. The modified protocols can be tested on other *Fragaria* cultivars and further can be used for cryopreservation of genetic resources of *Fragaria*.
