**2.1 Biological material**

Avocado seeds were obtained from two varieties of fruit. *P. americana var. drymifolia* were obtained from crops in Tingambato and local markets in Morelia, Michoacán, Mexico. *P. americana var. americana* seeds were obtained from local markets in Merida, Yucatán, Mexico. All fruits were in the ripe stage (**Figure 1**).
