**2.4** *In vitro* **germination conditions**

Sowing was carried out in 105 × 55 mm glass flasks with 25 ml of the medium, in a laminar flow hood with 10 individuals of *P. americana var. drymifolia* and 10 of *P. americana var. americana*. Once sown, the seeds were maintained in a culture chamber at a temperature of 25 ± 1°C with a photoperiod of 16 light hours at an intensity of 80 E·m-2·s-1 against 8 hours of darkness.

In Vitro *Seed Germination and Seedling Development of Two Avocado Varieties DOI: http://dx.doi.org/10.5772/intechopen.107005*

#### **Figure 1.**

*Characteristics of the avocado fruits in the mature consumption stage used in this work, Persea americana var. americana (A-C) and Persea americana var. drymifolia (D-F). The fruits were washed and separated into pericarp (A, D), mesocarp (B, E), and seeds (C, F). Scale line equivalent to 1 cm.*

#### **2.5 Determination of physio-morphological parameters**

The seeds were evaluated at 0, 1, 3, 5, 10, 15, 30, and 60 days, using the first evaluations (0, 1, 3, 5, 10, and 15 days) to determine the days of rupture and emergence of the seedlings, while 30 and 60 days were selected to determine four key parameters of the two varieties: percentage of germination, contamination, oxidation, and death or necrosis. Growth parameters such as seedling size, root length, stem and root diameter, and dry weight.
