**4.1 Silver nanoparticles**

These NPs can be loaded on different plant gum drug carriers for various anti-bacterial purposes. AgNPs have a strong bactericidal and catalytic effect according to various studies and they are extremely beneficial for preventing drug-resistant bacteria.

In a study, Gum acacia was loaded with silver NPs mixed with structures of HDN (fruit flavonoid). In this essay, after the preparation of GA-AgNPs (Gum acacia silver NPs loaded with NP structures of HDN), a Bactericidal assay was performed by incubating 108 colony-forming units per mL of MRSA and *E. coli* K1 with various concentrations of GA-AgNPs-HDN and respective controls in 1.5 mL centrifuge tubes at 37°C for 2 h. For negative controls, untreated bacterial cultures were incubated with PBS,7 while 100 μg/mL gentamicin-treated bacteria were used as positive control. The result for this essay is stated in **Table 2**.

In another study, gum Tragacanth was used as a drug carrier for Ag NPs. The welldiffusion method was used to study the antibacterial activity of the synthesized silver nanoparticles. Bacterial suspension was prepared by growing a single colony of Grampositive bacterial strain *S. aureus* and Gram-negative bacterial strains *E. coli* and *P. aeruginosa* overnight in nutrient broth and by adjusting the turbidity to 0.5 McFarland standard. Mueller Hinton agar plates were inoculated with this bacterial suspension,

<sup>7</sup> Phosphate buffer saline.

*Plant Gum Based Drug Carriers DOI: http://dx.doi.org/10.5772/intechopen.104399*


