**6. Conclusion**

The number of publications reporting new strategies for the obtention of nanocapsules for biomedical applications considerably rises every year. New materials

are being included for the preparation of nanocapsules, and their applications as stimuli-responsive systems, biosensors, and for theragnostic uses are being explored. The nanocapsule production method depends on the desired characteristics, the formulation materials, and the availability of laboratory equipment. Once the nanocapsules are fabricated, the protocol of characterization of the prepared systems is followed. In general, the nanoparticles described are spherical, with suitable sizes, size distributions, and zeta potentials according to their application, specifically, under 300 nm and with zeta potentials around ±30 mV. On the other hand, the activity of the nanoparticles loaded with the corresponding drug is tested *in vitro* in many of the works, although it is crucial to test that activity *in vivo* so as to access clinical study. For this reason, more and more *in vivo* studies appear, especially in the case of antitumoral therapy, which refers to the activity of the encapsulated drug and the ability of nanocapsules to release it in the desired target, avoiding side effects in healthy cells of the body.
