**Abstract**

Orchid is the most popular ornamental plant. One of the many orchid genus collected and known to have high morphological variations in the Liwa Botanical Garden is *Dendrobium*. But until now many collections have not been identified. This study aims to determine variations and identification of *Dendrobium* based on characters of morphological and stomata anatomical at Liwa Botanical Garden. Total collections of five *Dendrobium* accessions were namely CAT140, CAT 144, CAT 271, CAT 274, and IR015. The result showed leaf organs had high variations based on observation of 11 morphological characters. The phenetic relationship showed five accessions of *Dendrobium* can be classified into 2 main groups formed with a similarity index value of 0.813 based on the Gower similarity value and the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method. Meanwhile, the results of the observation of 9 anatomical characters on the upper and lower surfaces of the leaves showed accessions IR015, CAT 140, and CAT 274 have stomata only on the lower surface, while CAT 144 has stomata on the upper and lower surfaces. The results of this study are expected to provide basic information in identifying natural orchids and conservation efforts in Liwa Botanical Garden.

**Keywords:** anatomical, *Dendrobium*, Liwa Botanical Garden, morphological, UPGMA
