**3.2 Micropropagation**

Protocorm-like bodies (PLBs) are generically used in the micropropagation of *Phalaenopsis*. PLBs are cell masses similar to protocorm, which is the state of enlarged embryos during orchid seed germination. PLBs are somatic embryos induced from somatic cells of Orchids [21]. Since a PLB can form a number of new secondary PLBs on the surface by culturing on an appropriate medium, the proliferation efficiency is very high (**Figure 2**) and then they grow to plantlets. On the other hand, callus induction is difficult in *Phalaenopsis* Orchid and embryogenic callus (EC) induction was first reported by Sagawa [22]. Although studies on proliferation using, such as EC, have also been conducted [23–25], the methods using PLBs are still mainstream in *Phalaenopsis* micropropagation because PLB is easier to grow to plantlet than a callus. To date, PLB induction methods using a variety of plant tissue have been established, as shown in **Figure 3.**
