**Abstract**

*Phalaenopsis* orchids native to the tropics are called "Moth Orchids". It is one of the most commercially popular orchids because of its beautiful, colorful, and long-lasting variety of flowers. Biotechnology used in the production and breeding of *Phalaenopsis* was reviewed in this chapter. In the commercial production of *Phalaenopsis*, biotechnologies, such as methods of aseptic sowing and tissue culture, have been used for a long time. Recently, molecular phylogenetic analysis of original species and molecular breeding by the transformation of *Phalaenopsis* has been actively studied. The role of biotechnology in the *Phalaenopsis* orchid industry is significant, and the development of the technology in this field will bring further benefits to researchers, producers, and fancier of *Phalaenopsis* orchids.

**Keywords:** orchids, *Phalaenopsis*, classification, micropropagation, molecular breeding
