**3.4 Graphical presentation of cumulative data obtained from in vitro antioxidant assay**

From the above graph, it can easily assumed that the crystal compound may have great antioxidant activity. We know that lower the value of IC50 actually enhances the probability of having better antioxidant power of sample. When performing DPPH free radical scavenging activity, the sample had shown a very low value (4.1 *μg=ml*Þ of IC50 close to standard catechin (3.2 *μg=ml*), which ultimately provided a confirmation about the antioxidant capability of plant material.

Like DPPH test, the hydroxyl free radical scavenging assay and superoxide radical scavenging assay also convey messages regarding the free radical scavenging power and oxidant neutralizing potency of plant materials or samples. The IC50 value for hydroxyl radical scavenging assay of crystal compound was found as 35.01 ð*μg=ml*), which was near the value of standard catechin of 31.77 ð Þ *μg=ml* ; similarly the IC50 value was found as 29.04 and 26.79 for crystal compound and standard catechin, respectively, during super oxide radical scavenging assay (**Figure 7**).
