**2.5 Calculating the Rf value of crystal**

In TLC study, the retention factor has been utilized to screen and analyze the phytocompounds. The Rf value is a ratio of the distance moved by the compound and

**Figure 5.** *TLC of crystal compound (CC) after spraying vanillin-sulfuric acid spray reagent.* the distance moved by the solvent. The obtained Rf value for crystal compound was found as 0.43.
