**6.12 Antifungal activity**

The antifungal activity of *A. aspera* was obtained by using aqueous & ethanolic extract of root of its. Clotrimazole (1% w/w) used as standard drug for the comparison of *A. aspera* extract. The study is carried out to determine the antifungal activity of the *A. aspera* root extract by agar well diffusion method [60]. Antifungal activity was obtained through the sabouraud dextrose agar medium against culture of *Trichophyton rubrum*. The microorganism containing culture was inoculated and these inoculated plates were maintained for 2 hours at room temperature to allow diffusion of the solution into the medium. These petridishes incubated 25° ± 1 for 7 days [61]. Antifungal activity determined through the inhibition of diameters of zone surrounding each of the walls. The result has

shown that the zone of inhibition of ethanolic extract is 23 mm and zone of inhibition of aqueous extract is 19 mm taking 10 mg/ml of extract. Ethanolic extract of *Achyranthesaspera* shows good antifungal activity against *Trychophyton rubrum* than the aqueous extract [62].
