*1.2.5 Perspective regarding antioxidant*

I have tried my level best to isolate pure compound from black pepper and investigated a lot to identify antioxidant activity of that pure compound through a variety of in vitro evaluation processes.

The main target of any type of antioxidant or reductant is unpaired electron containing free radicals. Free radicals are a group of unstable chemicals, which can readily bind with biological components to be stable by gaining electrons resulting in destructive damage of cellular materials [28].

Typically, free radicals are formed inside the human body as a result of casual metabolic process, which can be both beneficial and harmful for human physiology; however, uncontrolled level of free radical production may initiate different types of diseases [29].

Antioxidant serves a prominent role in guarding our body from disease by neutralizing the series of oxidative stress produced by ROS [30]. During my research, my thought was to explore the free radical scavenging activity of sample and measure the extend of antioxidant power of that plant material as recent investigations have revealed that the plant-derived antioxidants with free-radical scavenging properties may have great therapeutic significance in diseases mediated by the free radicals such as neurodegenerative disease, autoimmune disorder, inflammatory disorder, cancer, and so on. To get relieved from these diseases, a variety of synthetic drugs are available in the market; however, these drugs may have some unavoidable side effects also. If people can put more concern on plant-based antioxidants, they will definitely get expected pharmacological effect with less or no side effects [31].
