**6. Future approaches and applications of miRNAs**

Identifying gene targets in human in a timely manner using plant miRNAs offers a reliable, effective, and economical way to determine the possible effects of interactions given by exogenous molecules, regardless of their origin, in human mRNA, studies have focused on providing information of importance for future projects or research, as in 2017, Kumar et al. [41] presented a cross-kingdom bioinformatics analysis, showing the importance of some plant miRNAs, coming from the plant *Camptotheca acuminata* commonly known as Happy Tree, with anticancer attributions, showing in their results, a strong association with several cancer pathways in human, and a possible important role in the regulation of complex disease networks for humans. Similarly, another plant *Ocimum bascilicum*, used for its therapeutic properties, underwent a bioinformatics analysis, by Patel et al. in 2019 [42], finding a close relationship of plant miRNAs with various genes involved in signaling pathways, various functional processes, and several organs in human. Not only have predictive studies been performed in plants that are used for their medicinal properties, there are also analyses where foods of daily consumption have shown interesting results as in the year 2020, the authors Rakhmetullina et al. [43] searched for target genes in human for 227 miRNAs of the Oryza sativa (rice) plant, finding 942 possible genes, which represent 5.4% of the total number of human genes studied, being this of major importance, due to the fact that four miRNAs present (osa-miR2102-5p, osa-miR5075-3p, osa-miR2097-5p, and osa-miR2919) were associated with a greater number of target genes, and since miRNAs can reach some tissues, and the circulatory system through ingestion, some targets were related to some biological processes involved in the development of cardiovascular and neurodegenerative diseases, the authors emphasize the importance of continuing to study them. Likewise, in 2022, Sánchez-Romo et al. [44] determined through bioinformatics analysis the possible mRNA targets in humans, resulting in 787 different genes for 84 miRNAs of the plant Triticum aestivum (wheat), showing some genes involved in cancer processes, risk of dementia and schizophrenia. In addition, functional enrichment analysis highlighted some pathways such as the Fanconi anemia pathway, circadian rhythm, and the dopaminergic synapse pathway, related to attention deficit hyperactivity disorder ADHD.
