**Additional information**

Parts of this book chapter were initially presented in the Master thesis of Max Mario Fuhlendorf available on the UFABC library platform, titled Loci image generator and the evolution of trypanosomatid ecotin: customized software as a tool for evolutionary analysis, 2018, Programa de pós-graduação em Evolução e Diversidade. The thesis has not been peer-reviewed and published.

*New Advances in Neglected Tropical Diseases*
