*Vector Control: Insights Arising from the Post-Genomics Findings on Insects' Reproductive Biology DOI: http://dx.doi.org/10.5772/intechopen.106273*

ligand remains to be further defined. Upon insemination, females are thought to mate once in their lifetime. In most *Anopheles* species*,* this is enforced by the formation of a mating plug that forms a barrier to prevent further female insemination [83]. The mating plug is composed of seminal secretions produced by the male accessory glands [84], and 20-hydroxyecdysone (20E) embedded is thought to play a signaling role in inducing monogamy in the female [26]. While a mating plug is not formed in *Aedes,* a physical barrier is temporarily formed by components of the male sperm produced at the male accessory gland [85]. Later, bioactive proteins collectively known as matrone can modulate female behavior at the neuronal levels and induce monogamy [86, 87]. Nevertheless, the exact molecular composition of matrone remains to be defined. A further understanding of the molecular basis for male-induced monogamy is of great importance, as it could potentially identify chemicals that could be used (e.g., in aerosols) to prevent virgin female mating.
