**Figure 2.**

*Next generation sequencing (NGS) is a group of technologies designed to sequence large numbers of DNA segments massively and in parallel, in less time and at a lower cost per base. The methodological approach can be summarized in five steps: 1) segmentation of the DNA into several fragments, 2) labeling of the DNA by means of primers or adapters that indicate the starting point for replication, 3) amplification of the DNA fragments labeled with adapters by methods based on polymerase chain reaction, 4) sequencing or reading of the DNA fragments, and 5) reconstruction of the complete sequence by means of reference sequences and export to data storage files.*

*Metabarcoding and Digital PCR (dPCR): Application in the Study of Neglected Tropical Diseases DOI: http://dx.doi.org/10.5772/intechopen.106272*

indels. Ignoring common single nucleotide polymorphisms and short insertions or deletions (indels), there are typically about 300,000 unique variants left to consider (**Figure 2**) [31, 35, 36].
