**Figure 4.**

*Digital droplet polymerase chain reaction (ddPCR) is a highly sensitive quantitative polymerase chain reaction (PCR) method based on fractionation of the sample into thousands of individual nano-sized water-in-oil reactions. Recently, ddPCR has become one of the most accurate and sensitive instruments for DNA detection. Consists of simple steps to follow: 1) prepare PCR-ready samples before starting ddPCR, 2) droplet generation, 3) droplet amplification by PCR; 4) Reading of drops, and 5) analysis of results. Positive droplets, which contain at least one copy of the target, exhibit greater fluorescence than negative droplets.*

*Metabarcoding and Digital PCR (dPCR): Application in the Study of Neglected Tropical Diseases DOI: http://dx.doi.org/10.5772/intechopen.106272*
