**2. Methods**

The present research work was carried out in the district of San Juan de Huasahuasi, located 48 km from the district of Tarma, at an altitude of 2,751 m above sea level. The raw material consists of mycorrhiza and rhizobium isolated from pea root and pine fungus, rhizobium is diazotrophic bacteria that have the ability to fix N in plant nodules and mycorrhizae are the double absorption organs that are formed when symbiont fungi live inside healthy absorption organs (roots, rhizomes, or stems) [7, 12, 21]. Black soil and potato peel bran were used as inputs, oil and potato dextrose agar were also used, the equipment used was a microorganism incubator and an analytical balance (**Figure 4**) [22–26].

Vegetable crops were sampled—potatoes, peas, and barley in the Huasahuasi district of Tarma province.

Among the methods used were the Association of Analytical Communities *in vitro* sowing method and colony counting. Once the product was obtained, a physicalchemical characterisation was carried out, evaluating fertility, antagonistic capacity, and biotisation (**Figure 5**).

The experimental process consists of obtaining strains of microorganisms and the biofertiliser mycorrhiza and rhizobium from pea root and pine fungus through two stages:

**Figure 4.** *Elements of biofertiliser.*

*Biotisation of Vegetables DOI: http://dx.doi.org/10.5772/intechopen.102551*

**Figure 7.** *Second stage: obtaining the optimal biofertiliser formula.*

#### **Figure 8.** *Inoculation of biofertiliser formulation to pea seed.*

The first stage consists of obtaining strains of mycorrhiza and rhizobium microorganisms, obtained from pea root and pine fungus, which is described in **Figure 6**.

The second stage consists of obtaining the optimal biofertiliser formulation of mycorrhiza and rhizobium, which is shown in **Figures 7** and **8**.


*F = Biofertiliser formula; F1 = 100 g of strains of microorganisms + 500 g of black soil and 200 g of potato peel bran; F2 = 200 g of strains of microorganisms + 500 g of black soil and 200 g of potato peel grist; F3 = 300 g of strains of microorganisms + 500 g of black soil + 200 g of potato peel bran; C = Vegetable cultivation (potato, pea, and barley); C1 = Potato; C2 = Peas; and C3 = Barley.*

#### **Table 1.**

*Relationship of biofertiliser formulation and plant cultivation.*

The statistical evaluation assessed the percentage effect of the biofertiliser in relation to its antagonistic capacity and biotisation, applying a completely randomised design (CRD) with the factorial arrangement and two replications [22], the factors were as follows:

Factor A: Inoculation of mycorrhiza and rhizobium biofertiliser formulation (F1, F2, and F3).

Factor B: Vegetable crops (potato, pea, and barley).

With a factorial arrangement of 3A × 3B = nine treatments and two replicates, the antagonistic and biotic effect of mycorrhiza and rhizobium fertiliser on vegetable crops (potato, pea and barley) is compared (**Table 1**).
