**3. Illustrative examples of information acquired**

Electron microscopy (EM) is a method to obtain biological and non-biological samples' images with a high-resolution. This method is frequently employed in biomedical research to examine the detailed structure of tissues, cells, organelles, and macromolecular complexes. High-resolution EM images are produced from the use of electrons having very short wavelengths as the illumination source. EM is used in conjunction with numerous additional methods (e.g., thin cutting, immune labeling, and negative staining) to answer specific questions. EM images can deliver crucial information about the structural and morphological basis of bone. Several results of prior studies that investigated bone with EM are presented in **Table 1**.


#### **Table 1.**

*Characterization of bone structure by SEM and TEM.*
