*5.1.4 Biological Samples*

Biological samples are among the most important groups to be examined in scanning electron microscopy. These samples show some differences from the samples from other areas. This difference is due to the fact that it is a living tissue and requires different pre-processing before imaging. Chief among these,

**Figure 11.** *Images of coated and uncoated samples.*

**A. Fixation:** The fixation time and temperature are different from tissue to tissue.


In **Figure 12** [10], we see a photo of a wet sample with moisture taken in SEM. Here we see a much clearer view of the sample dried with the critical dryer. This shows how important the sample preparation process is.
