**5. Conclusion**

Undeniably, the AFM power to measure topography, morphology, adhesion forces, elastic modulus, dielectric properties and energy dissipation characteristics via minimal invasion. Furthermore, the 3-Dimentional multi-parameter function provide information add-on in cases of trace fusion imaging. Considering the practicality, sampling and sample logistics are still remains desirable in AFM, though with respect to SEM the tedious work of sample preparation as well as high vacuum settings are not required. AFM has its advantages while studying, optimizing, understanding and validating techniques for examination of trace evidences found at the scene of crime. Also, microtraces evidences physiochemical features imaging can be done which can assist in classification and comparison. Though it has such advantages, roughness of substrate do hamper one or other way while studying the sample height measurements. Certain researchers have answer to this solution by accompanying surface roughness along with larger scan areas in supplementary phase imaging. In practice, AFMs can image rough surfaces as long as the roughness does not surpass the limit of scanner in vertical, *Z*-direction. But the probe will crash if the surface roughness surpasses the scanning limit. To grip the forensic applicability of AFM in real case work superfluous research as well as laboratory and crime scene authentication studies are prerequisite. AFM could yield surplus possibilities for forensic association and reconstruction, assisting in forensic analysis at activity level.
