**5. Conclusions**

Imaging at the nanoscale is very important to analyze the quality and structure of bone morphology. This review examines the images produced by electron microscopy of the femur bones of rats under sham conditions and osteoporosis due to ovariectomy. The scanned electron microscopy image with the help of the ImageJ application provides information that the femur bones of ovariectomized rats show signs of osteoporosis. Some of the parameters that characterize the cavities in the ovariectomized femur appear wider, with the edges of the cavity appearing to be cracked. In addition, the particle diameter also increased by an average of 77.16%. Therefore, electron microscopy is one of the best approaches, which can directly provide ultrastructural evidence for bone morphology, and furthermore, the results of this bone morphology analysis can provide insight into the characteristics and processes of osteoporosis.
