2.**Method B – Sterilisation and Cultivation process of Stevia, Chicory, Bee Balm (Cambridge Scarlet), and Rhodiola krylovii Cells.**

The 600 ml of M and S media with Kinetin was prepared using the above 11 reagents for plant cells. A total of 7 ml of solid media was poured into 80 12 test tubes. At the same time, sterile Psetri dishes were used to sterilise seeds. Seeds were sterilised using 70% ethanol for 2 mins. The rinsing of seeds was done with sterile water for 1 min. Seeds were washed with NaOCl for 1 min. Then, rinsing of seeds with sterile water for 1 min. The seeds were allowed to dry for a few mins. Two seeds were put in each test tube (20) for each type of plant and well labelled. The samples were placed in the climate chamber for incubation. Then, the test tubes containing samples were placed in the climate chamber and incubated under conditions for incubation with 16 hours of light phase and 8 hours of no light phase and temperature 250C ( 1°C) and relative humidity of 50%. After 14 days, the percentage of contamination was determined.

II.Using 0.9 g of agar in the solid media (the amount of agar was increased from 0.8 grams to 0.9 grams to reach average thickness).
