**6. Conclusion**

In a nutshell, the *in vitro* process of cultivating medicinal plants is a very tedious but delicate process that involves time, energy, proper care of the working area, and controlled environmental factors, such as temperature, humidity, moisture, and control equipment, which may alter the growing process and incur microbial contamination of plants. Thus, it was imperative to have studied the various sterilisation methods (A, B, C, and D) and the most effective method for the respective plants was used in this experiment since individual plant species have a particular method of

sterilisation that can yield good results during *in vitro* propagation of these plants. Although the issue of contamination cannot be avoided entirely during the sterilisation and cultivation process of medicinal plants, it can be significantly reduced if the parameters (pH, concentration 3% and temperature of around 10°C and 11°C) of the sterilising agent, such as NaOCl, are maintained in the required form and the pH of the media should be adjusted to around 5.8 [110]. To improve these medicinal plants' production yield and the sterilisation, cultivation process of Bee balm and Rhodiola krylovii should be studied for further research. The bioactive compounds of Chicory, Stevia, Beebalm, and *Rhodiola krylovii* produced using methods C and D, which had less percentage contamination, should be compared to the bioactive compounds of another sterilisation and cultivation method that might be used in growing the same plants elsewhere.
