**3.4 Preparation of cell suspension cultures**

Two factors were used to determine the growth of callus in cell suspension (liquid media), and these are the use of the growth regulator kinetin and the absence of growth regulator – Kinetin in the media with the aid of microscopy results were obtained (see **Figures 16** and **17**).

#### **3.5 Microscopy results of callus of chicory**

a. The cells were viewed under the microscope after 1 week of culturing the callus

See **Figure 18**.

**Figure 16.** *Callus of chicory in liquid media of + kinetin and – Kinetin.*

**Figure 17.** *Callus of chicory in the cell suspension.*

**Figure 18.** *(i) Chicory cells in liquid media (+kinetin). (ii) chicory cells in liquid media ( kinetin).*
