1.**Method C – Sterilisation and Cultivation Process of Stevia, Chicory, Bee Balm (Cambridge Scarlet), and Rhodiola krylovii Cells.**

The 400 ml of M and S media with Kinetin was prepared using the above reagents for plant cell culture. A total of 9 ml of media was poured into 40 sterilised test tubes. Sterile Petri dishes were used for the sterilisation process of seeds. Seeds were thoroughly washed in flowing water. Afterwards, seeds were sterilised with 2% carboxylic acid amide (CAA) fungicide for 2 mins, followed by washing seeds with 70% ethanol for 2 mins, then 3% NaOCl solution for 2 mins. Rinsing seeds thoroughly with sterile water thrice (three times) in three separate sterilised Petri dishes for 5 mins. Two seeds of Chicory and Stevia were put into 10 different test tubes containing solid media, respectively. Then, samples were placed in the climate chamber for incubation with no light intensity, temperature 250C ( 1 °C), and relative humidity of 50%. After 14 days, the percentage of contamination was determined.
