**4.2 Primers, PCR conditions and sequencing of ITS region**

The extracted DNA samples were amplified with PCR reaction for ITS regions. The forward primer: ITS1-5.8S (5′-GGAAGTAAAAGTCGTAACAAGG-3′) and the reverse primer rDNA-ITS4 (TCCTCCGCTTATTGATATGC) were used [70]. PCRs were performed in 25 μl reaction volume containing 12.5 μl master mix, 1 μl ITS1, 1 μl ITS4, 9.5 μl Nuclease free water and 1 μl templet DNA (100 ng/μl). PCR products were visualized in 2 % agarose gel, dyed with ethidium bromide and the photograph was taken using a Gel documentation system (Dynamica, GelView Master). The conditions for PCR reaction was: initial denaturation for 5 min at 95° C, followed by 34 cycles at 95° C for 30s, at 55° C for 1 min and at 72° C for 1 min and then final elongation at 72° C for 6 min. The amplified products were stored at −20° C. PCR products were sequenced using ITS1 primer via commercial outsourcing at Macrogen, Korea via Biotech concern. Finaly, Sequence data were imported by Chromas Software version 2. Sequence data were analyzed by BLAST program (Basic Local Alignment Search Tool) and GenBank (https://blast.ncbi.nlm.nih.gov/Blast.cgi).
