**3.3 Assay procedure for fumonisins detection**

200 μL conjugate solution was pipetted into dilution wells with 100 μL of each standard and sample extract. The mixture was mixed well and 100 μL of the mixture (conjugate and standard or samples) was transferred into antibody-coated wells. The plate was then incubated for 15 min with slow shaking and then washed with distilled water for 5 times. The plate was then tap dried. 100 μL of substrate solution was pipetted into antibody coated wells. The plate was incubated with shaking for 5 min. 100 μL of stop solution was pipetted into antibody coated wells. The absorbance of each well was read at 450 nm with a differential filter at 630 nm.
