*5.1.1.2 Larvicidal activity*

The larvicidal activity of nano-biopesticides can be measured by the leaf disc method by introducing them into the leaf, and concentrations of larvae can be determined after 96 hours. Some plants show larvicidal activity, such as leaf extract of *Ambrosia arborescens* which is helpful for larvae of some pests in concentrations such as LC50 = 0.28 ppm and LC90 = 0.43 ppm [92].
