*5.1.1.3 Anti-feeding activity*

The anti-feeding activity of nano-biopesticides can be measured by applying them to the leaf disc of pest food. The one-third-instar larva is introduced to the leaf, and the condemnations of leaf eaten by larvae can be measured every 24 hours. Anti-feeding activity has been observed about 92.4% in *Helicoverpa armigera* by applying silver-based nano-biopesticides [93].
