**6.3 Media milling method**

In this method, the plant extracts are exposed to an ultra-fine grinding medium, which results in the production of extract particles of a nanometer or smaller diameter. As a consequence of the contact of extracted particles with the milling medium, higher energy shear forces are produced throughout the milling process. This provides the required energy input to induce the microparticles to burst into nano-particles during the operation. For many days, milling material, which may consist of extract, a stabilizer, and water or another appropriate buffer, is rotated at a faster speed than the rest of the milling chamber and spun at a slower speed than the rest of the milling chamber [102].
