**10. Stepwise methodology for the calculation of radiation absorbed dose using OLINDA/EXM1 software**

A. Step-1

Analyze the series of patient images, times at which the images are acquired at various time point are calculated. These time points can be calculated simply by spreadsheet program by just subtracting one time from another.

B. Step-2

IA and IP were calculated as explained above.

C. Step-3

The system calibration factor "C" can be calculated as explained in Calibration Factor paragraph.

D. Step-4

Activity in the source organs and fraction of administered activity in the source organs were calculated. The percentage of administered activity (% IA) at each time point of images of various organs and tumors were calculated and used in OLINDA/EXM software for absorbed dose calculation. E. Step-5

Total number of disintegrations of source organs can be calculated with the help of percentage of injected activity at various time points. Depending on the data collected i.e. % IA of organs, mono-exponential or bi-exponential curve can be fitted in kinetic input form of OLINDA/EXM software and total number of disintegration can be calculated selecting the particular organs. For tumor and pituitary organs "remainder body" was selected and curve was fitted for the calculation of total number of disintegration (cumulated activity).

#### **Figure 8.**

*The selection of radionuclide in OLINDA EXM 1 software.*

#### **Figure 9.**

*The selection of models in OLINDA EXM 1 software.*
