**7. Future prospective**

To improve vegetables, certain factors must be considered such as an increase in production and insect resistance; abiotic stress tolerability; improved shelf life; processing quality; and improved nutritional contents, to name a few. In traditional breeding, it is difficult and time-consuming to achieve the stated traits. A novel genome editing technology, CRISPR/Cas9 technology can alter a plant genome resulting in several mutations. By putting the Cas9 gene into sgRNA specific viral

DNA, the plant has evolved virus resistance through CRISPR/Cas9 driven mechanisms [111]. A broad range of viral resistance plants was created by introducing several sgRNAs that target genomic areas of the whole virus into plants. CRISPR/ Cas9 can therefore be used to enhance metabolic engineering of horticultural crops by providing health-promoting factors.
