**1. Introduction**

Vegetable growing is a fundamental component of the economic system, which efforts to produce horticultural crops such as vegetables, fruits and spices, tubers, and medicinal plants. A substantial part of food and nutritional security is provided by these crops [1]. As the world's population grows, we need to increase agricultural output to maintain a sustainable food supply. The development of next-generation crops plays a significant role since conventional breeding techniques have been extensively used and time-consuming [2, 3]. Transgenesis is the possible alternate plant breeding approach where public acceptance is a significant concern in terms of commercialization. Recently, recombinant DNA technology using nucleases such as ZFNs [4], TALENs [5, 6], and CRISPR/Cas9 [7] has proven to be a viable technique to modify the targeted location in the genome and is widely utilized in many agricultural crops. The technology is expected to be widely used shortly.
