**Herbs/Spices Compound name Structure** Estragole Celery leaves Limonene b-selinene Clove oil Eugenyl acetate Cumin Cuminaldehyde b-pinene Curry leaf Carbazole

#### *Structure: Activity and Emerging Applications of Spices and Herbs DOI: http://dx.doi.org/10.5772/intechopen.99661*

#### *Structure: Activity and Emerging Applications of Spices and Herbs DOI: http://dx.doi.org/10.5772/intechopen.99661*

**Table 5.** *Structures of bioactive compounds in herbs and spices (source: [5, 27]).*

#### **5. Innovative functionality and medical application of herbs and spices**

#### **5.1 Antibacterial and antifungal activities**

Phytochemicals such as phenolics, carotenoids and organosulphur compounds present in herbs and spices function for antimicrobial activity. Clove, oregano, thyme, cinnamon, cumin, rosemary, garlic, ginger, basil, fennel, coriander and galangal have been reported for antibacterial and antifungal activity [29]. Antimicrobial activity of clove bud oils possesses antibacterial and antifungal properties owing to the high level of eugenol present in the essential oil [30]. Curry leaves also exert antifungal activity [31]. Coriander and cinnamon were also reported for antifungal activity against *Aspergillus parasiticus, Cladosporium cladosporoides, Eurotium herboriorum, Penicillum chrysogenum* and *Aspergillus carbonarius* [32]. Galangal is a food additive used in Thailand and some other Asian countries and has been reported for antibacterial activity against *Staphylococcus aureus* and *Listeria monocytogenes.* The synergistic effect of galangal, rosemary and lemon iron bark was also reported [29]. Extracts of roselle, clove and rosemary were reported for antibacterial activity against *Escherichia coli, Vibrio parahaemolyticus, Pseudomonas aeruginosa, Salmonella enteritidis, Bacillus cereus, Candida albicans* and *S. aureus* [33].

#### **5.2 Antiviral activity**

A number of herbs and spices have been reported for antiviral activity [34]. Antiviral activity of turmeric was observed and documented in different viruses such as hepatitis virus, corona virus, influenza, Human Immunodeficiency virus (HIV) and others [34, 35]. Turmeric targets different cellular pathways, which further inhibiting the growth, and replication of viruses and this makes it an ideal candidate as an anti-viral drug. Antiviral activity of curcumin was observed against different viruses including hepatitis viruses, SARS coronavirus, influenza viruses, human immunodeficiency virus (HIV), herpes simplex virus, dengue virus and chikungunya virus [36]. Utomo et al. [37] reported that curcumin binds and inhibits the target receptors including SARS-CoV-2 protease, spike glycoprotein-RBD, and PD-ACE2, which are involved in virus infection.

Garlic produces an enzyme called alliinase and this enzyme has antiviral activities. Antiviral activity of garlic extract has been studied against influenza virusA/H1N1 in cell culture and it was found that it inhibits the virus penetration and proliferation in cell culture [38]. In another study conducted by Shojai et al. [39], garlic extract showed inhibitory activity on infectious bronchitis virus in

chicken embryo. During the inception of COVID-19 pandemic, Ministry of AYUSH, India released an advisory of immunity and self-care of Ayurveda's spices [40]. This includes the use of spices such as turmeric, cumin, coriander, and garlic that are recommended in cooking. They have also advised taking herbal drinks, tea/decoction (kadha) made from basil, cinnamon, black pepper, ginger, and raisin once or twice in a day [40].

#### **5.3 Anticancer activity**

National Foundation for Cancer Research [41] mentions that herbs and spices are effective in the preventions and treatment of cancer. For decades, the National Cancer Institute and Memorial Sloan-Kettering Cancer Center in the United States have recognised the cancer preventive potential of herbs and spices from labiatae, zingiberaceae, and the unbelliferae family [10]. Some of the reported culinary herbs and spices for anticancer activities include basil, caraway, cardamom, clove, cumin, dill garlic, ginger, rosemary, saffron and thyme [42]. Likewise, Wigutow [43] reported turmeric, ginger, cayenne pepper, saffron, oregano and garlic to be one of the good cancer fighters. Epidemiological studies suggest that a high intake of raw and cooked garlic may provide a protective effect against stomach and colorectal cancers [44]. A commonly available spice, Saffron (*Crocus sativus* L.), used as a food flavouring spice has also been thoroughly reported for its cancer preventive and tumoricidal properties [45]. Research over the last 50 years indicated that curcumin, a polyphenolic ingredient in turmeric, can prevent cancer, and can be used to treat cancer [46]. Flaxseeds also known as linseeds, usually sprinkled on cereals, salads and desserts as nutritional booster is also a good source of lignans known as "phytoestrogens", which are studied for chemo-preventive properties. Various studies have shown that garlic and organosulfur compounds, especially diallyl disulfide, can slow the development of cancers of the skin, oesophagus, stomach, colon, liver, lung and the mammary glands [47].

#### **5.4 Antidiabetic activity**

In all cases of diabetes, development of one or more complicated chronic diseases such as neuropathy, retinopathy, nephropathy and cardiomyopathy are common. Culinary herbs and spices have been found useful in the treatment of diabetes [48]. Curcumin, an active component of turmeric, was reported to ameliorate diabetic nephropathy instreptozotocin-induced diabetic rats [49]. Supplementation of turmeric in patients with-ßovert type 2 diabetic nephropathy was also shown to attenuate proteinuria, IL-8 and can be administered as a safe adjuvant for these patients [50]. Cinnamon was also reported for its hypoglycaemic activity [51]. In addition, aniseed, bay leaf, cardamom, cinnamon, cumin, dill, ginger, hops, rosemary, saffron, sage have been examined in type 2 diabetes mellitus patients [52].

#### **5.5 Antioxidant activity**

Antioxidants can protect lipids and oils in food against oxidative degradation. When added to food, antioxidants control rancidity development, retard the formation of toxic oxidation products, maintain nutritional quality, and extend the shelf-life of products [27]. Natural antioxidants contained in spices help to reduce oxidative stress. Bakheit and Foda [53] determined the antioxidant activity of individual spices like black pepper, black cumin, and clove using (DPPH) free radical scavenging assay. Rosemary is one of the most effective spices widely used in food processing. It is the only spice commercially available for use as an antioxidant in

Europe and around the world [54]. Garlic has also been reported for antioxidant activity [55]. It has been documented by Wootton-Beard and Ryan [56] that natural antioxidant compounds are much effective than the synthetic compounds with regards to toxicity and carcinogenity. Antioxidants help to defend human bodies by deterring the formation of free radicals chain reaction. Moreover, antioxidant compounds are responsible for preventing diseases including cancer, cardiovascular disease, Alzheimer's disease, and muscular degeneration [56].

#### **5.6 Hypocholesterolemic activity**

Hypercholesterolemia, commonly known as high blood cholesterol, is a major risk factor for the development of atherosclerosis and occlusive vascular disorders [57]. Therapeutic lifestyles such as low saturated fat and cholesterol diet, weight management, and increased physical activity are vital for blood cholesterol regulation. Spices and herbs have also been reported for cholesterol-lowering activity [58]. Scientific evidence from several animal models revealed that curcumin from turmeric and capsaicin from red pepper are potent hypocholesterolaemic and hypolipidemic agents [59, 60]. Spices have been shown to possess good nutrient benefits with low calories, possess good inhibitory profiles on carbohydrate modulating enzymes, ACE and HMG-CoA reductase, which correlates to their total phenolic contents, phenolic profile and antioxidant properties. Ahmed et al. [61] reported that spices belonging to the Apiaceae family, for example, cumin, coriander, fennel and dill, are rich in monosaturated fatty acids and contain a good amount of polysaturated fatty acids and thus have great hypocholesterolemic effect.

#### **5.7 Anti-inflammatory activity**

Different studies have demonstrated an association between the typical Western diet rich in refined starches, sugars, saturated and transfatty acids but poor in fruit, vegetables, fibre, *ω*-omega-3 fatty acids and whole grains, which causes an increased tendency toward inflammatory disorders and related diseases, such as cardiovascular diseases, arthritis, or diabetes [62]. These signs can be prevented by eating or cooking food with herbs and spices that have medicinal properties to combat inflammation. Prevention is better than cure and healthy eating should be promoted to prevent inflammatory symptoms [63]. That is why turmeric is traditionally used in India in the treatment of rheumatic disorder because of its antiinflammatory properties [64]. Ginger also has anti-inflammatory effect and has been researched to be effective in ameliorating arthritis pain [65]. Other herbs and spices reported for anti-inflammatory potential are cinnamon, garlic, black pepper and clove [63]. Herbs and spices have also been reported as anti-inflammatory food supplements against COVID-19 as excellent sources of vitamin C, for example, thyme, coriander, turmeric and cardamom [66].

#### **6. Recent application of herbs and spices**

#### **6.1 Flavouring**

Although herbs and spices are low-cost commodities, they have been valued as gold or jewels for many centuries being utilised as food additives all over the world. Herbs and spices are basically used in enhancing the flavour of food [13]. The addition of herbs and spices to vegetables, salad and fruits can help to improve the

taste. Herbs and spices can be used as substitute for the less desirable taste promoters such as salt, sugar and fat. They generally add to appearance and smell of food [5]. African dishes such as jollof rice, soups and snacks as well as some drinks are flavoured using herbs and/or spices such as onion, chilli, turmeric, ginger, clove and tamarind [67]. Herbs such as basil, celery leaf, cumin, garlic also are important in African traditional snacks [5].

#### **6.2 Preservatives**

Green preservatives are becoming increasingly popular with new techniques of applications being developed [68]. Herbs and spices have been used to fortify foods throughout history not only as flavours but also as preservatives. Though used to enhance the organoleptic properties of food, they are also used in controlling natural spoilage by decreasing or eliminating foodborne pathogens thus increasing the shelf life [69]. Phenolic compounds present in herbs and spices are a major constituent responsible for their antimicrobial activity thus leading to preservation [1]. Chives, garlic, celery seed, tarragon, dill, black cumin, cinnamon, turmeric, cumin all have compounds functioning as preservatives in them [70]. Gallic acid, rutin and caffeic acid in black mustard, alicin and diallylsulfides in chives, garlic and eugenol in cinnamon prevent the growth of *E. coli* [71–73]. Capsicum in chilli pepper inhibits the growth of *Salmonella typhimurium* [74]. The antimicrobial efficacy of hydrosols of thyme, bay leaf, rosemary, sage and black cumin against *S. typhimurium* and *E. coli* has been reported [75]. Rosemary integration to rice cakes effectively inhibited *Bacillus cereus* and *S. aureus* [76]. The preserving function of herbs and spices can be linked to their antioxidant and antimicrobial activities [70, 77].

#### **6.3 Dairy products**

Antimicrobial properties of herbs and spices can be successfully used to control the growth of spoilage and pathogenic bacteria in dairy products. Phenolic compounds such as tea catechins, oleuropein, ferulic acid, ellagic acid and coumaric acid have been found to prevent the growth of some pathogenic bacteria (*S. aureus, S. enteritidis and L. monocytogenes*) and fungi [78]. Bakrm and Salihin [79] reported that addition of *Cinnamonum verum* and *Allium sativum* water extract in goat, cow and camel milk had no important effect on the acidification through fermentation. However, the presence of these two herbs in milk improved the proteolytic activity of the used cultures with the highest proteolytic activity gotten in cow milk yoghurt. Herbs and spices are also added to cheeses to impart unique flavours. These cheeses are regularly considered as specialty cheeses. Most spices impart specific flavours to cheeses, and some may affect the microbiological quality. Hamid and Abdelrahman [80] investigated the effect of adding 0.02% Cinnamon, Cardamom and Fenugreek powder to goat's milk curd after coagulation on the quality of the obtained white soft cheese. The additions of these spices enhanced the flavour and odour of goat's milk cheese. Herbs and spices also improved biological value and prolonged the shelf life of cottage cheese [80]. Black pepper, black cumin, and clove were also used to produce novel Mudaffara cheese [53]. Herbs and spices fortify dairy product acting as natural antioxidant, biopreservative, improvement of sensory qualities while also functioning for nutritional and medicinal purposes [13]. Herbs-fortified dairy products are a good source of antioxidant [81].

#### **6.4 Essential in water and drink fortification**

The production of flavoured and fortified water is another area in which herbs and spices have found application [82]. Asides the regular function of water for distribution of nutrient and maintenance of electrolyte balance, functional water (flavoured and fortified) has added medicinal functions of preventing illness and diseases. Herbs alongside vitamins, minerals, and amino acids are the main components of these beverages [12]. Beet root, ginger, and red ginseng have been used in fortification of quite a number of beverages and drinks [83, 84].

#### **6.5 Herbal cosmetics**

Exposure of human skin to sunlight and other atmospheric conditions causes production of reactive oxygen species, which can react with DNA, proteins, and fatty acids, causing oxidative damage and impairment of antioxidant system. Beauty products made from herbs are tested and preferred for low toxicity, mildness, moisturising, and cleaning activities. Herbal cosmetics have recently gained ground and natural ingredients such as herbs and spices have been incorporated into skin care formulation either directly or their essential oils [15]. Botanical extracts are multifunctional in nature because they possess various properties like photoprotection, antiaging, moisturising, antioxidant, astringent, anti-irritant, and antimicrobial activity, which are correlated with each other [85]. Turmeric is effective for the treatment as well as prevention of psoriasis and other skin conditions such as acne, eczema, sun damage, wounds and premature ageing since it inhibits the activity of phosphorylase kinase [86]. Garlic possess anti-inflammatory effect which reduces the inflammation of acne. Garlic oils are also useful in controlling sores, pimples and acne [87]. Eugenol present in clove is useful in keeping the skin dirt-free also reduces redness and inflammation of acne. Ginger possesses cleansing and antiseptic compounds in it and thus keep the skin smooth and without blemish and is used in many cosmetics for skin health [15].

#### **6.6 Pest control**

A number of herbs and spices have been reported for insecticidal potential and essential oil from Cinnamon is an example of such. Extracted oil from stem bark, leaves and fruits of *Cinnamomum camphora* were found to possess great fumigant toxicity against *Tribolium castaneum* and *Lasioderma serricone* [14]. Black pepper has also been reported for pest control. Scott et al. [88] reports that black pepper offers a unique biopesticide material that can be used in controlling small-scale insect outbreaks and also reduce the likelihood of development of resistance. Basil, sage and lemon thyme have all been researched for insecticidal activity [89].

#### **7. Current challenges in the use of herbs and spices**

Although several herbs and spices have been reported for great medicinal and flavouring benefits, there are a number of challenges confronting their use. The current challenges in the use of herbs and spices include toxicity, allergy as well as quality and adulteration.

#### **7.1 Adulteration**

The detection of adulteration in herbs and spices is important for value assessment in order to mitigate the health problems caused by undeclared constituents [90]. Adulteration can be direct and intentional which involves the practice of partially or fully substituted constituents with inferior ones. Adulteration can also be unintentional resulting mainly from the absence of a proper evaluation method and/or clerical errors [91]. Reported adulteration of herbs and spices include the addition of dried pawpaw seeds with black pepper, red beet pulp and tomato waste to pepper powder, exhausted clove added to good ones, grass seeds added to cumin seeds, exhausted ginger added to good ginger, capsicum added to ginger powder, wild *Curcuma* spp. added to turmeric powder and lots more [92].

Techniques have been developed to counter alteration including physical methods such as macroscopic and microscopic analysis [93] or analytical methods including chromatographic techniques, electrophoresis, spectroscopy, chemometrics and hyphenated techniques [94, 95]. Biotechnological [96] and hybridization methods, polymerase chain reaction, immunological and biosensors assays can all be used in adulterant detection [92].

#### **7.2 Toxicity and allergy**

Quite a number of studies have revealed the cytotoxicity, carcinogenicity, neurotoxicity, genotoxicity and teratogenicity of phytochemicals derived from herbs and spices [97]. Capsaicin has been reported to cause neural and retinal degeneration [98] and increase blood ATL level [99] in experimental animals. Chilli peppers was reported to cause hypertensive crisis in a 19 years old male [100]. Singh et al. [101] reported the cytotoxic effect of cinnamon extract on human and mouse cell lines. Eugenol at 0.06% concentration was also reported to have toxic effect on human skin cells [102]. Although herbs and spices have been reported for several health benefits, their use also has side effects just as possible in other medicinal plants [103]. Therefore, it is paramount that attention is paid to their dosage. Generally, herbs and spices should be consumed with the knowledge of their existing side effects [104]. As stated by Stiller [105], people's allergic reactions to spices and herbs can be either Type IV or Type I reactions. Occupational contact dermatitis is the most experienced Type IV allergy while rhinitis, bronchial asthma, gastrointestinal symptoms, oral allergy syndrome and anaphylactic shock are the Type I allergies most experienced.

#### **8. Conclusion**

Herbs and spices are products obtained from plants which have been used traditionally for their flavour enhancement properties and have also been scientifically proven to possess medicinal properties. Bioactive compounds present in these plants are responsible for their health benefits when they function for anti-oxidant, antiinflammatory, anticancer, antimicrobial and antiviral activities. Besides the general use of herbs and spices in flavouring dishes, they have also found application in enrichment of water, other drinks and dairy products. They have been used for preservation of food, production of skin care product and pesticides. It was also established that bioactive compounds such as eugenol, curcumin and essential oils of a variety of herbs and spices are of great important, however, there is a need for more in-vivo studies for better evaluation and toxicological properties of herbs and spices.

### **Author details**

Adeyemi Ojutalayo Adeeyo<sup>1</sup> \*, Tshianeo Mellda Ndou<sup>2</sup> , Mercy Adewumi Alabi3 , Hosana Dumisani Mkoyi<sup>4</sup> , Erinfolami Motunrayo Enitan<sup>5</sup> , Daniso Beswa<sup>6</sup> , Rachel Makungo<sup>7</sup> and John O. Odiyo<sup>8</sup>

1 Department of Ecology and Resource Management, Faculty of Sciences, Engineering and Agriculture, University of Venda, Thohoyandou, South Africa

2 School of Environmental Sciences, University of Venda, Thohoyandou, South Africa

3 Department of Microbiology, School of Sciences, Federal University of Technology, Akure, Nigeria

4 Department of Life and Consumer Sciences, College of Agriculture and Environmental Sciences, UNISA Science Campus, Florida, South Africa

5 Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa

6 Department of Biotechnology and Food Technology, University of Johannesburg, Johannesburg, South Africa

7 Department of Hydrology and Water Resources, Faculty of Sciences, Engineering and Agriculture, University of Venda, Thohoyandu, South Africa

8 Vaal University of Technology, South Africa

\*Address all correspondence to: firstrebby@gmail.com

© 2021 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

*Structure: Activity and Emerging Applications of Spices and Herbs DOI: http://dx.doi.org/10.5772/intechopen.99661*

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#### **Chapter 4**
