**2.1 The effect of substrate type and temperature on the activity of glucose isomerase**

In these experiments, either glucose or fructose was used as substrates of reactions at an amount of 1% w/v. The flasks were incubated in shakers at 60 or 30°C at 150 rpm. The pH values of media were 5.8 and 5.4 for glucose and fructose as substrates, respectively.
