**2. Materials and methods**

In this part, the "synthetic media" term was defined for different experiments that were in other parts, too. This term means that media are prepared with different wine components such as ethanol, glycerol, tartaric acid, calcium, and so on. To mimic the wine environment and activate the enzyme, 0.06 g MgSO4.7H2O was added into 100 mL synthetic media solutions [13]. The experiments were conducted with 1 g of immobilized glucose isomerase added to 100 mL of solutions.

*Determining Glucose Isomerase Activity in Different Wine Environments to Prevent Sluggish… DOI: http://dx.doi.org/10.5772/intechopen.100499*
