**2.4 Identification of selected plant growth promoting antagonistic bacterial isolates by sequence analyses of 16SrDNA**

#### *2.4.1 Extraction of genomic DNA*

Bacterial culture from NA media was transferred in LB broth and shaken for 18 h at 28°C. Then genomic DNA of antagonistic bacteria was extracted according to Wizard® Genomic DNA purification Kit (Promega, Madison, USA). Obtaining the DNA pellet was rehydrated by adding 25 μL DNA rehydration solution and kept it overnight at 4°C. Finally the genomic DNA samples of the isolates were preserved at 20°C for further use.
