**5.5 Eosin methylene blue agar**

The colonies of *E. coli* grow with a green metallic sheen, which is due to the metachromatic property of dyes (eosin and methylene blue in the ratio of 6:1) and the lactose fermenting property of *E. coli*, which changes the pH of the medium to acidic. Hence, making the medium more selective for *E. coli* makes the identification much more easier [30] as shown in **Figure 7**.
