**5. Conclusion**

Molecular diagnostic platforms have become promising alternatives to traditional methods for *E. coli* detection. In particular, LAMP assay and DNA biosensors because of their advantages of lower detection limits, and high reproducibility are preferred for pathogen detection. In this review, we have discussed the recent advances in the development of PCR, LAMP assay, and DNA biosensors platforms applied to *E. coli* detection. In the case of CRISPR/Cas platforms, the major challenge associated with the CRISPR/Cas sensing platform is the time taken to produce the results. Therefore, the future perspective would be to reduce the assay turnaround time for CRISPR/Cas sensing. Nonetheless, CRISPR/Cas sensing platforms possess the potential to overcome the use of conventional molecular diagnostic platforms and become a promising tool for next-generation diagnostic platforms for sensitive and selective detection of DNA in clinical, food, and environmental samples. In the future, more, specific, sensitive, cost-sensitive, and portable biosensors will be required to detect *E. coli*, hence, further leading to controlling and monitoring the waterborne epidemics.

## **Conflict of interest**

The authors declare no conflict of interest.

*Molecular Diagnostic Platforms for Specific Detection of* Escherichia coli *DOI: http://dx.doi.org/10.5772/intechopen.101554*
