**Abstract**

The unique diversity of microbes makes them ideal for biotechnological purposes. In this present study, 16 actinobacterial isolates were screened on media supplemented with Bisphenol A (BPA). Three out of 16 isolates exhibited high biocapacity to degrade BPA as *a* carbon source. Four different mixed actinobacterial consortia were developed using the above strains and the effect of each consortium on biomass growth; laccase production and BPA degradation were examined. At 100 mg/L BPA concentration, the three-member consortium grew well with maximum laccase activity as well as maximal degradation rate of Bisphenol A than the other two-member consortium. The consortium of *Actinomyces naeslundii, Actinomyces bovis*, and *Actinomyces israelii* degraded 93.1% with maximum laccase activity of 15.9 U/mL, followed by *A. naeslundii* and *A. israelii* with 87.3% and 9.5 U/mL. This was followed by *A. naeslundi* and *A. bovis* with 80.4% and 8.7 U/mL, while *A. bovis* and *A. israelii* degraded 76.0% with laccase activity of 7.0. The gas chromatography– mass spectrometry (GC–MS) analysis of biodegraded BPA showed the presence of oxalic acid and new products like 1,2,4-trimethylbenzene and 2,9-dimethyldecane.

**Keywords:** Bisphenol A, laccase, biodegradation, biocapacity, gas chromatography– mass spectrometry (GC–MS), *Actinomyces naeslundii*, *Actinomyces bovis*, *Actinomyces israelii*
