**3.10 Prediction activity spectra for substance (PASS)**

The biological activity of the selected drug-like candidates was then evaluated using PASS. It is well known that each substance has a wide spectrum of biological activities as evident from some new uses of many old drugs. This allows the tool to utilize this information to predict biological activities based on their probable activity (Pa) and probable inactivity (Pi). When Pa is greater than Pi (Pa > Pi), the compound is likely to possess the predicted biological activity [55, 56]. PASS predicted Karatavicinol, Marmin, Colladonin and Pectachol to be potential antileishmanial agents (**Table A5**). Colladonin showed the highest Pa of 0.768 and Pi of 0.006 followed by Taccalin (Pa of 0.711 and Pi of 0.009) and

Pectachol with a Pa of 0.694 and Pi of 0.009. Betaxanthin had no prediction as an antileishmanial agent.
