**6. Whey proteins role as anti-cancerous agents through production of inflammatory cytokines**

Cancer is associated with a high rate of morbidity and mortality. According to a worldwide study, 27 main cancers were responsible for millions of deaths per year [81]. Dietetic modification has been proposed as an encouraging prophylactic strategy. Metabolites that modulate biomarkers of cancer began to emerge. Whey protein seems to be effective in this respect, and the assumptions have been supported by some data. In vitro, isolates of whey proteins had an impact on melanoma B16F10 cells that is cytotoxic in nature, as shown by the release of high caspase-3 [83]. According to a study performed on the rats suffering from gut cancer, fed whey protein hydrolysate, there were less tumor foci [84]. Before and after chemotherapy, a cervical cancer patient received whey protein (10 g three times quotidian) and a monthly testosterone infusion, which increased body lean mass, physical movement, and life quality [85]. Lactoferrin has been shown in vivo to stimulate cytokines in the guts and suppressing cancer growth in colon. Research established that consuming 3.0 g of bovine lactoferrin on a regular basis enhance the IFN levels that reduces the propagation of colorectal polyps [86]. While the available evidence is limited and the conclusion is far from definitive, but it might play a role of foundation for further research regarding anticancer experiments [21].

## **7. Whey protein as immunomodulators through maintenance of inflammatory cytokines**

The complex network of lymphoid glands, effector cells, and molecules that make up the host immune defense is critical for survival [87]. Pathogenesis, metabolic, and degenerative disorders results as immunity lost. Dietary ingredients including probiotics, oligosaccharides, and β-glucans have been shown to have immune-boosting properties [88]. Whey proteins have been found to boost tolerance during childhood and thereby eliminate certain immune problems [89]. Whey proteins, upon digestion, found to activate variation among cells of immune system and recruitment of secondary lymphoid glands, according to a rodent report [89]. A comparative study among child's who fed whey incorporated formula were and the kids who consumed bovine milk, the chances of atopic dermatitis were 16 percent less in the former than later group [90]. Psoriasis patients' skin healed by taking 20 grams of whey protein isolate a day [21]. Although the psoriasis region and intensity score ranged from 4.9 to 28.8, it improved from 0.8 to 8.4 after 3 months of therapy. Higher glutathione levels and reduced systemic inflammation were linked to a reduction in autoimmune disorder symptoms [91].
