*6.5.4 Stable plurilamellar vesicles*

Stable plurilamellar vesicles (SPLVs) are prepared using method described by [37]. The phospholipid suspension is to be taken in round bottom flask and evaporated using rotary evaporator to form dried film. To the dried phospholipid suspension an aqueous phase (HEPES [N-2-hydroxyethylpiperazine-N9–2 ethanesulfonic acid] buffer) was added and mixed. The mixture is to be shaken in mechanical shaker and placed in bath sonicator with a nitrogen gas passed through it to facilitate evaporation during the sonication. The liposomes are to be suspended again in the HEPES buffer. The elimination of the nonencapsulated material was done by column filtration and three washes needed in HEPES buffer.
