**4.1 Preparation of inclusion complexes**

Once the CD-NS is ready, the inclusion complex can be obtained by several procedures [55] as its summarized in **Figure 2**. Firstly, it is performed the classical mixing of drug and CD-NS in a solvent, commonly water for 24 h to form the complexes. In this procedure, we should consider the insoluble or soluble nature of our complexes. Depending on this, the desire fraction is purified. Using kneading, the drug and the CD-NS are mixed in a mortar with an appropriate quantity of water or another solvent to form the complexes. At this point, other additional techniques are used to form the powder: The solvent can be removed using lyophilization, coevaporation, or spray-drying procedures.
