**7.4 Super critical reverse phase evaporation (SCRPE)**

This is single step process for preparation of liposomes under supercritical carbon dioxide condition [57]. Liposomal dispersion is formed by emulsion formation with water mixed in ethanol, LR-dipalmitoylphosphatidylcholine, and supercritical carbon dioxide at stirring condition with required pressure. Transmission electron microscopy (TEM) results showed large unilamellar vesicles formed at size range 0.1 to 0.2 μm [57]. The entrapment efficiency results also showed that five times more drug was entrapped by this method compared with Bangham method [57]. Results showed SCRPE is one best technique with single step for large single lamellar vesicles with good entrapment efficiency [58, 59].

## **7.5 Modified ethanol injection method**

Novel approaches are available for liposomes production with principle of ethanol injection which are namely cross flow-injection technique [60–63], microfluidic channel method [64–66] and membrane contactor method [67].
