**7.1 Heating method**

A new heating method was available for production of liposomes [53]. All materials are to be mixed in aqueous phase and then to it glycerol (3% v/v) is to be added which will increase the stability for lipid vesicles. The glycerol containing lipid mixture will be heated up to 120° C to form vesicles. The studies on TLC of lipids showed that there is no degradation at mentioned temperature [54].

## **7.2 Spray-drying**

Spray-drying of lipid mixture and drug is one of the methods for large scale industry production. The lecithin and mannitol are to be dissolved in chloroform,

#### *Drug Delivery through Liposomes DOI: http://dx.doi.org/10.5772/intechopen.97727*

sonicated in bath sonicator and spray dried using mini spray dryer to form dried liposomal vesicles. Spray-drying conditions of temperatures 120° C for inlet and 80°C for outlet and 1000 ml/hr. of air flow rate are to be maintained. The obtained spray dried product is to be rehydrated using aqueous phase [55]. The liposomes size depends on used aqueous phase volume for rehydration [55].
