*6.6.1 Detergent dialysis*

In liposomal preparations some bile salts and alkyl glycosides are used as detergents for solubilization of lipids in micellar systems. The shape, size of liposomal vesicles always depends on used detergent chemical nature, concentration and lipids [38–40]. The common procedures for removing detergent from micelles are by dilution method [41, 42], gel chromatography [43] and dialysis by hollow fibers [44] or membrane filters [45, 46]. By combining ethanol injection and cross flow injection a new technique was developed to form proteo liposomes [47]. Liposomes of size range 40–180 nm is seen when detergent solubilises lipids to yield micelles [48]. Other methods like calcium induced fusion [49], nanoprecipitation [50] and emulsion techniques [51, 52] are used for liposomes preparation. These classical methods need more solvents and very harmful to human health, also require complete removal of organic solvent.
