**6. Conclusions**

As the findings of non-invasive PGT-A (NIPGT-A) research have been discussed, and researchers have noticed that the success rate and accuracy of the test are closely related to the types of culture medium, culturing and methods, sampling method, detection platform s and gold-standard reference used of detection. This chapter systematically expounds on how researchers use foetal free DNA for non-invasive PGT-A detection and thoroughly analyses the factors affecting its accuracy, possible problems and future application prospects. We hope that it serves as a good reference for non-invasive PGT-A being widely used in standardized operations before clinical application.

## **Acknowledgements**

This work was supported by the National Key Research and Development Program (Grant No. 2018YFC1003100).

#### **Conflict of interest**

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest.

#### **Acronyms and abbreviations**


*Non-Invasive Chromosome Screening for Embryo Preimplantation Using Cell-Free DNA DOI: http://dx.doi.org/10.5772/intechopen.98416*

