**Abstract**

Honey bee venom, known as apitoxin, is composed of several peptides, the most important of which is melittin. This peptide is a current focus of research since it can improve the immune system and act against cancer due to its anti-mutagenic, anti-inflammatory, and even contraceptive effects. This makes it very desirable to obtain melittin-producing bacteria, and for this reason, this study has aimed at the cloning of *Escherichia coli* with the melittin gene from western bee. In order to do this, the total RNA of the western honey bee (*Apis mellifera*) has been extracted, and a reverse transcription polymerase chain reaction (RTPCR) has been carried out, at different annealing temperatures (68.0, 68.2, 68.4, 68.6, 68.8, and 69.0°C) to amplify the melittin cDNA. The annealing temperature of 68.4°C has allowed the highest production. Subsequently, this cDNA has been cloned into the pGEM-T vector, which has transformed *E. coli* JM109. This transformation has been corroborated by the blue/white test mediated by X-gal.

**Keywords:** *Apis mellifera*, *E. coli*, melittin, expression vector, transformation
