*2.4.4 Ion exchange chromatography*

This technique was used to purify the isoenzyme of the LOX.

• DEAE-Cellulose (A50) was prepared by dissolving 20 gm of DAEA-Cellulose A50 in 250 mL of Buffer phosphate pH 7, leaving the solution suspended for 24 hours and at 4° C. The solution was switched several times from time to

time to remove the soft minutes from the suspended solution until the pH reaches 7.

