*2.4.2 Dialysis*

The process of dialysis for the dissolved protein was done to remove the ammonium sulfate residues that were used to precipitate the proteins, using a dialysis bag. The dissolved protein was added into the bag and immersed in the buffer solution (Buffer phosphate (0.001 M) pH 7). This process was carried out for 24 hours, with the solution being changed periodically. This step of purification was done at 4° C to maintain the activity of the enzyme. The activity and protein concentration of the enzyme were measured after the end of the process.
