*2.4.5 Measuring GGT activity in blood serum*

The Szasz method [27] was used to measure the effectiveness of the GGT enzyme, and the reaction equation is shown in Eq. (1):

$$\begin{aligned} \text{L}-\gamma-\text{Glutamul}-\text{3}-\text{carbonay}-\text{4}-\text{nitroonilid}+\text{Glycylglycin} & \stackrel{\gamma\text{-GT}}{\Rightarrow} \text{L}-\gamma\\ -\text{Glutamul}-\text{Glycylglycin}+\text{5}-\text{Amino}-\text{2}-\text{nitroo}-\text{benzoat} & \end{aligned} \tag{1}$$

In Eq. (1) the reaction of measured the effectiveness of the GGT.

The activity of the enzyme is directly proportional to the formation of 5-amino-2-nitro-benzoate at a wavelength of 405 nm.
