**8. Evaluation of the MeOH extract of** *Galphimia glauca* **in Behavioral models of anxiety**

The anxiolytic and anti-depressive effects were evaluated for the *G. glauca* MeOH extract (wild material) standardized with content of G-B (8.3 mg/g), using the elevated light–dark labyrinth and forced swimming in albino (ICR) mice. The extract, administered orally, three times (24, 18, and 1 h prior to the test) at doses of 125, 250, 500, 1,000, and 2,000 mg/kg was capable of significantly increasing (*p* < 0.05) the number of entries, as well as time spent on the elevated labyrinth's open arms, which indicates an anti-anxiolytic effect. A similar effect was observed in the light–dark paradigm test: time spent in the light box increased in treated mice. However, this treatment was not able to change any parameter in the forced swimming test [148].
