**4. Tissue processing**

After digestion, cells are strained by strainer to separate the debris from it. Then, cells micro clumps are washed with PBS or HBSS twice or tricefollowed by centrifugation [54]. Cell pellet collected from centrifugation is suspended in 2 ml of culture media. Count the viable cell by hemocytometer or by tryptophan dye exclusion method [55]. Cell viability also can be measured by the intracellular adenosine triphosphate levels which are commercially available kit [56]. Immunohistochemistry and immunofluorescence techniques used to localize, identify and quantitate the cells based on cell surface marker [57].
