**6. Application of cell culture**

Nowadays application of cell culture is exceptionally essential in life science and medical science. Cell culture technique is an excellent tool its applications are [1, 39, 40] as a). Production of pharmaceutical biochemicals b). Embryological study (CEF cell culture) c). Recombinant biomaterials (rDNA) and vaccine manufacturing, testing of the drug, drug sensitivity, and cytotoxicity of cell d). Production of human and animal vaccines (primary chicken fibroblast cells e). Manufacturing of immunotherapy f). Production of different enzymes, hormones (synthetic), immunobiological (like monoclonal antibodies, interleukins, lymphokines), and anticancer agents g). Cell culture is an excellent way to teach cell biology study h). Production of agricultural products like milk, (cultured) meat, fragrances i). In the microbiological study (Virus propagation, virology) j). Genetic engineers and biotechnologist are utilizing it within their field of research k). The aging, toxic compound study, cell morphology, cell physiology, and the study of mutagenesis cell culture have great impact.
