**3.4 CFU-F assay**

CFU-F assay can be used *in vitro* to evaluate the proliferation potential of MSCs. CFU-F assay is a well-established method for the quality control of MSCs' preparation. This section describes a traditional assay for CFU-F to evaluate the colony forming ability of human MSCs.


*Isolation and Expansion of Mesenchymal Stem/Stromal Cells, Functional Assays and Long-Term… DOI: http://dx.doi.org/10.5772/intechopen.100286*

