**11. Conclusion**

In recent years' importance and the application of cell culture cannot explain in a word. Cell culture technology is an extensively accepted technique in the field of molecular biology study and life science research. Due to high feasibility, cell culture practices highly demandable in biopharmaceutical works. This document summarizes the theoretical background, basic concepts regarding cell culture (animal cells, BHK-21, CEF cell culture). The above discussion gives an ideal concept to understand the whole process of cell culture technique, its applications and it helps to build constructive problem-solving confidence related to cell culture along with inspiration. Conclusively, it is said that all the steps regarding cell culture give a fundamental idea of cell culture.
