**2. Cell culture**

General concept of cell culture is the propagation of cells or fibroblast or living tissues in a defined media that conducive their growth. Shortly, a growth of cell artificially known as cell culture. CEF (Chicken Embryo Fibroblast) cell culture is the culture of fibroblast cells obtained from embryo. Embryonated eggs commonly used in the production of bulk antigens, vaccines and other biochemical. SPF (Specific Pathogen Free) eggs obtained from SPF (Specific Pathogen Free) chicken flocks which have been intensively monitored for infectious agents and have not been vaccinated; or, where justified (e.g. for production of some inactivated vaccines) and in line with the marketing authorization, from healthy chicken flocks. For the propagation of virus laboratory personnel or researcher should have to choose specific route of inoculation of the SPF egg based on the study microorganisms (virus) that is being propagated [4].

Baby Hamster Kidney cells (BHK-21) are generally used in life science research work and the biopharmaceutical industry. Chinese hamster ovary (CHO) cells, baby hamster kidney (BHK) cells, mouse myeloma cells comprising NS0 and SP2/0, hybridomas, and human cell lines (HEK293, HT-1080) are the very commonly used mammalian cell lines at large scale [5]. Among these cell lines, BHK-21 has found applications at large scale in veterinary viral vaccines against foot and mouth
