*High-Throughput Single Extracellular Vesicle Profiling DOI: http://dx.doi.org/10.5772/intechopen.97544*

and particle size distribution are analyzed in AFM [2]. AFM tip indents the EVs and records the force-distance curve to provide insight into the mechanical properties, including mechanical stiffness, Young's modulus and bending modulus [4]. Tumorexosomes of bladder cancer cell lines were proven to show differential mechanical properties dependent on the malignant state [5]. Although constrained in the throughput of EV analysis, the precise analysis of structural and biophysical properties via AFM provides unique measures in EV engineering for targeted delivery and therapeutic (**Figure 2**) [4].
