**Abstract**

Among all nanostructured materials, nanofibers (NFs) are the one class that is widely used in tissue engineering (TE) and regenerative medicine (RM) areas. NFs can be produced by a variety of different methods, so they can be used almost for any tissue engineering process with appropriate modifications. Also, the variety of materials that can form nanofibers, production methods, and application fields increase the value of NFs greatly. They are almost suitable for any tissue engineering applications due to their tunable properties. Hopefully, this chapter will provide brief information about the production methods (electrospinning, wet spinning, drawing, etc.), characterization methods (Scanning Electron Microscopy, Transmission Electron Microscopy, Atomic Force Microscopy, etc.), and tissue engineering applications (core-shell fibers, antibacterial fibers, nanoparticleincorporated fibers, drug-loaded fibers, etc.) of NFs.

**Keywords:** nanomaterials, nanofibers, production, characterization, biomedical applications, regenerative medicine, tissue engineering
